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PE anti-mouse CD194 (CCR4) Antibody

Pricing & Availability
Clone
2G12 (See other available formats)
Regulatory Status
RUO
Other Names
CC CKR-4, CHEMR1, Cmkbr4, LESTR, MGC151418, Sdf1r
Isotype
Armenian Hamster IgG
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Product Citations
publications
2G12_PE_102108.jpg
Hyper-immunized BALB/c splenocytes stained with GK1.5 (CD4) APC and 2G12 PE
  • 2G12_PE_102108.jpg
    Hyper-immunized BALB/c splenocytes stained with GK1.5 (CD4) APC and 2G12 PE
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131203 25 µg £100
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131204 100 µg £234
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Description

Mouse CCR4 cDNA contains 1531 bp, and encodes a protein of 360 amino acids that is 85% identical to human CCR4. CCR4 binds CCL17 (TARG) and CCL22 (MDC). Naïve T cells, bearing receptors for cutaneous antigens, become activated in skin-draining lymph nodes and express cutaneous lymphocyte antigen (CLA), which confers to these cells the capacity to migrate into the skin to exert their normal effector functions (1). CCR4 and CCR10 play an important role in the ligand-mediated recruitment of T cells into the skin in mice and humans, specifically with regards to tethering, firm adhesion, and subsequent extravasation to the site of injury (2,3). CCR4 is expressed in cutaneous regulatory T cells (Tregs). These cells are crucial for the induction and maintenance of self-tolerance and are present in peripheral tissues such as skin and gut under normal, noninflamed conditions (4).In addition, recruitment of Foxp3+ T regulatory cells mediating allograft tolerance depends on the CCR4 chemokine receptor and its ligand CCL22 (5).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Saito K, et al. 2008. J. Immunol. 181:6889. PubMed
  2. Ueha S, et al. 2007. J. Leukoc. Biol. 82:1230. PubMed
  3. 3. Sharma R, et al. 2009 J. Immunol. 183:1065 (FC) PubMed
  4. Dogan R, et al. 2011. J. Leukoc. Biol. 89:93. PubMed
  5. Liu H, et al. 2011. Sci Transl Med. 3:82. PubMed.
  6. Shankar SP, et al. 2012. J Immunol. 188:6347. PubMed.
Product Citations
  1. Alam A, et al. 2022. Cancer Cell. 40:153. PubMed
  2. Lin G, et al. 2022. Int J Immunopathol Pharmacol. 36:3946320221117933. PubMed
  3. Zhang W, et al. 2023. Front Pharmacol. 14:1092767. PubMed
  4. Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed
  5. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  6. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  7. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  8. Wiesner D, et al. 2016. J Immunol. 196: 365 - 374. PubMed
  9. Loebbermann J, et al. 2013. Proc Natl Acad Sci U S A. 110:2987. PubMed
  10. Honjo A, et al. 2013. Respir Investig. 51:241. PubMed
  11. Zhong W, et al. 2022. Nat Commun. 13:4390. PubMed
  12. Morimura S, et al. 2021. Int J Mol Sci. :22. PubMed
  13. Meadows D, et al. 2015. PLoS One. 10: 0143738. PubMed
  14. Liu Y, et al. 2013. Cancer Res. 73:550. PubMed
  15. Suga H, et al. 2013. J Immunol. 190:6059. PubMed
  16. Ohsugi T, Kumasaka T 2011. PLoS One. 6:e18518. PubMed
  17. Rashid MH, et al. 2021. Oncol Rep. 45:1171. PubMed
  18. Zhu X et al. 2019. Cell reports. 27(4):1205-1220 . PubMed
  19. Engel I, et al. 2016. Nat Immunol. 10.1038/ni.3437. PubMed
  20. Lind L, et al. 2021. J Neurovirol. 27:145. PubMed
  21. Lee H, et al. 2010. J Immunol. 185:6698. PubMed
  22. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  23. Spath S, et al. 2022. iScience. 25:104998. PubMed
  24. Moriwaki A, et al. 2011. Am J Respir Cell Mol Biol. 44:448. PubMed
RRID
AB_1236367 (BioLegend Cat. No. 131203)
AB_1236367 (BioLegend Cat. No. 131204)

Antigen Details

Structure
GPCR, chemokine receptor, seven transmembrane receptor
Distribution

Memory T cells, macrophages, platelets, basophils, Th2 cells, and Tregs

Function
CCR4 and its ligands (CCL17 and CCL22) are important for the recruitment of memory T cells into the skin in various cutaneous immune diseases.
Interaction
Interaction between CCR4 and its ligand TARC/CCL17 on activated endothelial cells mediates T cell extravasation by stimulating integrin-dependent adhesion
Ligand/Receptor
CCl17 and CCL22
Cell Type
Basophils, Embryonic Stem Cells, Macrophages, Platelets, T cells, Th2, Tregs
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Biederman T, et al. 2002. Eur. J. Immun. 32:3171.
2. Mirshahpanah P, et al. 2008. Exp. Dermatol. 17:30.
3. Kusumoto M, et al. 2007. J. Interferon Cytokine Res 27:901.
4. Clark RA and Kupper TS. 2006. Blood 109:194.
5. Lee I, et al. 2005. J. Exp. Med. 201:1037.

Gene ID
12773 View all products for this Gene ID
UniProt
View information about CD194 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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