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PE anti-mouse CD197 (CCR7) Antibody

Pricing & Availability
Clone
4B12 (See other available formats)
Regulatory Status
RUO
Other Names
C-C chemokine receptor type 7 (CCR7), MIP-3 beta receptor, EBV-induced G protein coupled receptor 1, EBI-1, CD197
Isotype
Rat IgG2a, κ
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Product Citations
publications
CCR7vsCD44_041505
C57BL/6 splenocytes stained with CCR7-PE (clone 4B12) and CD44-FITC (gated on CD3+ cell population)
  • CCR7vsCD44_041505
    C57BL/6 splenocytes stained with CCR7-PE (clone 4B12) and CD44-FITC (gated on CD3+ cell population)
  • RIgG2aPEvsCD44_F_in_T_041505
    C57BL/6 splenocytes stained with CCR7-PE (clone 4B12) and CD44-FITC (gated on CD3+ cell population)
Compare all formats See PE spectral data Supplemental Data...
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120105 50 µg £126
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120106 200 µg £292
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Description

CD197 is also known as C-C chemokine receptor 7 (CCR7) or EBI-1. CCR7 is a G-coupled rhodopsin-like member of the GPCR superfamily with a predicted molecular weight of 43 kD that is expressed on hematopoietic stem cells, most naive T cells, some memory T cells, B subset, and mature dendritic cells. CCR7 is a receptor for the chemokines CCL19 (MIP3 beta) and SLC (6CKine, Exodus-2, TCA-4, CCL21) that plays a role in thymocytes development, T cell adhesion at intestinal sites, the homeostatic recirculation of memory T cells, and chemotaxis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CCR7 transfected RBL-2H3 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The 4B12 antibody does not inhibit binding of ligand to receptor. Additional reported applications (for the relevant formats) include: immunoprecipitation. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302) is recommended prior to immunofluorescent staining.

Staining with clone 4B12 is recommended at 37°C (see supplemental data of PE staining at differing temperatures).

Application References

(PubMed link indicates BioLegend citation)
  1. Ohl L, et al. 2004. Immunity 21:279.
  2. Ritter U, et al. 2004. J. Leukocyte Biol. 76:472.
  3. Lan YY, et al. 2005. Am. J. Transplant. 5:2649. (FC)
  4. Lee JH, et al. 2007. J. Immunol. 178:301. (FC) PubMed
  5. Kurooka M and Kaneda Y. 2007. Cancer Res. 67:227. (FC) PubMed
  6. Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC)
  7. Sakai N, et al. 2006. P. Natl. Acad. Sci. USA 103:14098. (FC)
  8. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC)
  9. Hwang IY, et al. 2007. J. Immunol. 179:439. (FC) PubMed
  10. Kang SG, et al. 2007. J. Immunol. 179:3724.
  11. Mao A et al. 2005. J. Immunol. 175:5146. PubMed
  12. Allende ML, et al. 2008. FASEB J. 22:307. PubMed
  13. Kang SG, et al. 2007. J. Immunol. 179:3724. PubMed
  14. Chen H, et al. 2005. J. Immunol. 175:591. PubMed
  15. Florido M, et al. 2009. Immunobiology. 214:643. PubMed
  16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  17. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Product Citations
  1. Lin C et al. 2019. Immunity. 50(1):137-151 . PubMed
  2. Liu J, et al. 2019. Immunity. 50:600. PubMed
  3. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  4. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  5. Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed
  6. Alcaraz-Serna A, et al. 2021. Sci Adv. 7:. PubMed
  7. Chen H, et al. 2005. J Immunol. 175:591. PubMed
  8. Shi B, et al. 2018. J Immunol. 200:586. PubMed
  9. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  10. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  11. Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed
  12. Lutes LK, et al. 2021. eLife. 10:00. PubMed
  13. Silva M, et al. 2021. Sci Immunol. 6:eabf1152. PubMed
  14. Jin X, et al. 2020. Cell Reports. 31(8):107690. PubMed
  15. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  16. Hwang I, Kehrl C 2004. J Immunol. 179:439. PubMed
  17. Schilling B, et al. 2014. Ann Oncol. 25:747. PubMed
  18. Kuhn NF, et al. 2020. Nat Commun. 4.74375. PubMed
  19. Wang HB, et al. 2017. Allergy. 72:927. PubMed
  20. Espinosa JR, et al. 2018. Front Immunol. 9:1371. PubMed
  21. Sakurai T, et al. 2021. Life Sci Alliance. 4:. PubMed
  22. Flórido M, et al. 2009. Immunobiology. 214:643. PubMed
  23. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
  24. Zhou X, et al. 2022. Front Pharmacol. 13:872188. PubMed
  25. Galle-Treger L, et al. 2020. J Allergy Clin Immunol. 145:502. PubMed
  26. Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
  27. Chatterjee S et al. 2017. Cell metabolism. 27(1):85-100 . PubMed
  28. Benhamron S, et al. 2012. PLoS One. 7:e35602. PubMed
  29. Zhang Q, et al. 2021. Front Cell Dev Biol. 9:655552. PubMed
  30. Dong X, et al. 2022. Front Immunol. 13:896472. PubMed
  31. Fukushima K, et al. 2020. Immunity. 52(3):542-556. PubMed
  32. Yashiro T, et al. 2020. FEBS Open Bio. 10:1115. PubMed
  33. Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed
  34. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  35. Chakraborty P, et al. 2020. Cell Reports. 28(7):1879-1893.e7.. PubMed
  36. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  37. Hsu HP, et al. 2021. J Biol Chem. 296:100419. PubMed
  38. Szeto C, et al. 2022. Nat Commun. 13:4951. PubMed
  39. Liang Z, et al. 2022. iScience. 25:105233. PubMed
  40. Wirasinha RC, et al. 2021. J Exp Med. 218: . PubMed
  41. Allie S, et al. 2011. J Immunol. 186:6280. PubMed
  42. Lim J, et al. 2010. J Immunol. 185:4022. PubMed
  43. Guenther C, et al. 2019. Front Immunol. 10:1138. PubMed
RRID
AB_389357 (BioLegend Cat. No. 120105)
AB_389357 (BioLegend Cat. No. 120106)

Antigen Details

Structure
Rhodopsin-like GPCR superfamily, G-protein coupled receptor 1 family, integral membrane protein, predicted molecular weight 43 kD
Distribution

Hematopoietic stem cells, T subsets, mature dendritic cells

Function
Homozygous mutation. Receptor for MIP-3 beta and SLC chemokines. Probable mediator of EBV effects on B lymphocytes. Plays a role in T cell adhesion at intestinal sites; may also play a role in the homeostatic recirculation of memory T cells and chemotaxis.
Ligand/Receptor
MIP3 beta, SLC (6CKine, Exodus-2, TCA-4)
Cell Type
Hematopoietic stem and progenitors, T cells, Dendritic cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Schweickart VL, et al. 1994. Genomics 23:643.
2. Yoshida R, et al. 1998. J. Biol. Chem. 273:7118.
3. Campbell JJ, et al. 1998. J. Cell Biol. 141:1053.
4. Willimann K, et al. 1998. Eur. J. Immunol. 28:2025.

Gene ID
12775 View all products for this Gene ID
UniProt
View information about CD197 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 3    Revision Date: 12/05/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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