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PE anti-mouse CD1d Antibody

Pricing & Availability
Clone
K253 (See other available formats)
Regulatory Status
RUO
Other Names
Antigen-presenting glycoprotein CD1d1, CD1.1, Ly-38
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
K253_PE_082610
C57BL/6 splenocytes stained with K253 PE
  • K253_PE_082610
    C57BL/6 splenocytes stained with K253 PE
See PE spectral data
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140805 50 µg £68
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Description

CD1d is a type I transmembrane protein and member of the MHC family, with a molecular weight ranging from 43-49 kD, depending on the glycosylation degree. CD1d is expressed by antigen presenting cells such as dendritic cells, monocytes, macrophages and B cells; also expressed by thymocytes and intestinal epithelial cells. CD1d present glycolipids to iNKT cells that recognize them by their Vα14i TCR.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
α-GalCer:mCD1d complexes coupled to Mycobacterium tuberculosis PPD
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: ELISA1, immunofluorescence2 to detect CD1d in the lipid rafts on the cell membrane, and block1 CD1d recognition of an iNKT cell hybridoma.

Application References

(PubMed link indicates BioLegend citation)
  1. Yu KO, et al. 2007. J. Immunol. Methods 323:11. (ELISA, Block)
  2. Im JS, et al. 2009. Immunity 30:888. (IF)
Product Citations
  1. Zhou Y, et al. 2021. Methods Mol Biol. 2388:35. PubMed
  2. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  3. Hanasoge Somasundara AV, et al. 2021. Cell Rep. 37:110099. PubMed
RRID
AB_10643277 (BioLegend Cat. No. 140805)

Antigen Details

Structure
Member of the MHC family. Type I transmembrane protein. Associates with β2-microglobulin. Molecular weight ranges from 43 - 49 kD, depending on glycosylation.
Distribution
Dendritic cells, monocytes, macrophages, B cells, thymocytes, intestinal epithelial cells
Function
Glycolipid presentation to iNKT cells
Ligand/Receptor
Vα14i TCR
Cell Type
Dendritic cells, Monocytes, Macrophages, B cells, T cells, Epithelial cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Arrenberg P, et al. 2010. P. Natl. Acad. Sci. USA 107:10984.
2. Mattarollo SR, et al. 2010. J. Immunol. 184:5663.
3. Wang J, et al. 2010. P. Natl. Acad. Sci. USA 107:1535.
4. Nieuwenhuis EE, et al. 2009. J. Clin. Invest. 119:1241.

Gene ID
12479 View all products for this Gene ID
UniProt
View information about CD1d on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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