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PE anti-mouse CD275 (B7-H2, B7-RP1, ICOS Ligand) Antibody

Pricing & Availability
Clone
HK5.3 (See other available formats)
Regulatory Status
RUO
Other Names
ICOS ligand, ICOSL, B7 homolog 2, B7-H2, B7H2, B7RP1, B7h, ICOS-L
Isotype
Rat IgG2a, κ
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Product Citations
publications
HK5dot3_PE_021808
C57Bl/6 mouse splenocytes stained with CD275 (clone HK5.3) PE
  • HK5dot3_PE_021808
    C57Bl/6 mouse splenocytes stained with CD275 (clone HK5.3) PE
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107405 50 µg £92
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Description

CD275 is a 40 kD protein, also known as ICOS ligand, B7-RP1, B7-H2, and B7h. It is a member of the immunoglobulin receptor superfamily expressed on splenic B cells, dendritic cells, and macrophages. The expression of this antigen is increased by TNF-α stimulation and is highly upregulated by local inflammation. B7RP1 binds to ICOS and co-stimulates T cell and B cell responses. Two isoforms of this protein have been reported to occur as a result of alternative splicing. These isoforms show differential tissue distribution.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse B7-RP1 transfected cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of ligand binding1,2. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 107405) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 107404) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 107408). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 107410) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Iwai H, et al. 2003. J. Immunol. 171:2848. (Block)
  2. Kohyama M, et al. 2004. P. Natl. Acad. Sci. USA 101:4192. (Block)
  3. Zhang P, et al. 2007. J.Immunol. 178:474. PubMed
  4. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Product Citations
  1. Li H, et al. 2022. Cancer Cell. 40:36. PubMed
  2. Peng C, et al. 2022. Immunity. 55:98. PubMed
  3. Molina MS, et al. 2022. PLoS One. 17:e0273075. PubMed
  4. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  5. Zhou J, et al. 2019. Immunity. 50:403. PubMed
  6. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
  7. Raineri D, et al. 2021. Biomedicines. 10:. PubMed
  8. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  9. Zhang H, et al. 2021. Cell Reports. 35(6):109096. PubMed
  10. Barbet G, et al. 2018. Immunity. 48:584. PubMed
  11. Molina MS, et al. 2021. Front Immunol. 12:699128. PubMed
  12. Niven J, et al. 2019. Cell Rep. 28:21. PubMed
  13. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
  14. Angulo G, et al. 2021. eLife. 10:00. PubMed
  15. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
RRID
AB_2248797 (BioLegend Cat. No. 107405)

Antigen Details

Structure
B7 Immunoglobulin superfamily, approximately 40 kD
Distribution

Isoform 1 has highest expression in lymphoid tissues, splenic B-cells, T-cells, dendritic cells and macrophages; lower levels in many non-lymphoid tissues. The expression of isoform 2 is restricted to heart, spleen, and kidney.

Function
Binds to ICOS. Co-stimulates T cell responses including proliferation and cytokine secretion. Stimulates B cell proliferation and differentiation to plasma cells.
Ligand/Receptor
ICOS
Cell Type
B cells, Dendritic cells, Lymphocytes, Macrophages, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Yoshinaga SK, et al. 1999. Nature 402:827.
2. Ling V, et al. 2000. J. Immunol. 164:1653.
3. Ling V, et al. 2001. J. Immunol. 164:1653.

Gene ID
50723 View all products for this Gene ID
UniProt
View information about CD275 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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