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PE anti-mouse CD59a Antibody

Pricing & Availability
Clone
mCD59.3 (See other available formats)
Regulatory Status
RUO
Other Names
MACIF, 1F-5Ag, H19, HRF20, MIRL, P-18, Protectin
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
mCD59.3_PE_120811
C57BL/6 mouse bone marrow cells were stained with CD59 (clone mCD59.3) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram). Data shown was gated on erythroid cell population.
  • mCD59.3_PE_120811
    C57BL/6 mouse bone marrow cells were stained with CD59 (clone mCD59.3) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram). Data shown was gated on erythroid cell population.
See PE spectral data
Cat # Size Price Quantity Check Availability Save
143103 25 µg £105
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143104 100 µg £247
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Description

CD59a, one of the two isoforms of CD59, is also known as membrane attack complex inhibition factor or MACIF. CD59a is a 18-25 kD GPI-anchored membrane glycoprotein of that protects cells from complement-mediated lysis by binding the complement factor C9, preventing its incorporation to the C5b-C8 complex, and impairing the formation of the membrane attack complex (MAC). CD59a is broadly expressed by different cell types, including endothelia, erythrocytes, and platelets.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
EL-4 cells transfected with murine CD59a
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting of mouse erythrocytes1.

Application References

(PubMed link indicates BioLegend citation)
  1. Harris CL, et al. 2003. Immunology 109:117. (WB)
Product Citations
  1. Janela B, et al. 2019. Immunity. 50:1069. PubMed
  2. Pais H, et al. 2019. Sci Rep. 9:5760. PubMed
RRID
AB_10962439 (BioLegend Cat. No. 143103)
AB_10962439 (BioLegend Cat. No. 143104)

Antigen Details

Structure
Glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein, 18-25 kD
Distribution
Different cell types, including endothelia, erythrocytes, and platelets
Function
Inhibits complement-mediated cell lysis by preventing the incorporation of factor C9 to the C5b-C8 complex, preventing the formation of the membrane attack complex (MAC)
Interaction
Complement factor C9
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Costimulatory Molecules
Molecular Family
CD Molecules
Antigen References

1. Pu JJ and Brodsky RA. 2011. Clin. Transl. Sci. 4:219.
2. Yu Q, et al. 2010. Cell. Mol. Immunol. 7:334.
3. Kohli P and Levy BD. 2009. Br. J. Pharmacol. 158:960.

Gene ID
12509 View all products for this Gene ID
UniProt
View information about CD59a on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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