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PE anti-mouse CD88 (C5aR) Antibody

Pricing & Availability
Clone
20/70 (See other available formats)
Regulatory Status
RUO
Other Names
C5a ligand, C5a anaphylatoxin receptor, C5AR, C5R1, C5AR1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
20slash70_PE_111709
C57BL/6 bone marrow cells stained with 20/70 PE and Ly-6G/Ly-6C (Gr-1) APC
  • 20slash70_PE_111709
    C57BL/6 bone marrow cells stained with 20/70 PE and Ly-6G/Ly-6C (Gr-1) APC
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135805 50 µg £100
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135806 200 µg £247
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Description

CD88 is a single chain protein with seven membrane-spanning regions and a molecular weight of 43 kD. CD88 is expressed by neutrophils, eosinophils, and monocytes. There have been reports of CD88 expression in non-immune cells such as glial cells, cerebellar granule cells, cardiomyocytes, and vascular endothelial cells. CD88 is coupled to heterotrimeric G proteins such as Gi, and after C5a binding, the signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA2, among other molecules, resulting in chemotaxis, granule enzyme release, and superoxide anion production.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
mC5aR transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications for the relevant formats include: blocking of C5a binding.

Application References

(PubMed link indicates BioLegend citation)
  1. Godau J, et al. 2004. J. Immunol. 173:3437.
  2. Shushakova N, et al. 2002. J. Clin. Invest. 110:1823.
  3. Kim SH, et al. 2013. Int Immunol. 25:623. PubMed
Product Citations
  1. Purvis GSD, et al. 2022. Br J Pharmacol. 179:2754. PubMed
  2. Leach SM, et al. 2020. Cell Rep. 33:108337. PubMed
  3. Mehta G, et al. 2015. J Immunol. 194:5446. PubMed
  4. Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed
  5. Monteran L, et al. 2022. Nat Commun. 13:5797. PubMed
  6. Kim SH, et al. 2021. Cell Reports. 35(2):108995. PubMed
  7. Medler TR et al. 2018. Cancer cell. 34(4):561-578 . PubMed
  8. Hoogendijk AJ, et al. 2020. Cell Reports. 29(8):2505-2519. PubMed
  9. Bosmann M, et al. 2012. J Immunol. 188:5086. PubMed
  10. Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed
  11. Kim S, et al. 2013. Int Immunol. 25:623. PubMed
  12. Taylor L, et al. 2016. PLoS One. 11: 0160685. PubMed
  13. Bisht K, et al. 2014. Biochem Biophys Res Commun. 449:94. PubMed
  14. Bosteels C, et al. 2020. Immunity. 52(6):1039-1056.e9.. PubMed
  15. Zheng QY, et al. 2020. FASEB J. 34:10590. PubMed
  16. Zheng QY, et al. 2019. Front Immunol. 10:1866. PubMed
  17. Giles DA, et al. 2018. J Clin Invest. 128:5322. PubMed
  18. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
RRID
AB_2067285 (BioLegend Cat. No. 135805)
AB_2067285 (BioLegend Cat. No. 135806)

Antigen Details

Structure
Single chain protein with seven membrane-spanning regions, 43 kD.
Distribution

Neutrophils, eosinophils, monocytes, glial cells, cerebellar granule cells, cardiomyocytes, and vascular endothelial cells.

Function
After C5a binding, the CD88 signal results in chemotaxis, granule enzyme release and superoxide anion production.
Interaction
CD88 is coupled to heterotrimeric G proteins such as Gi, The signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA2, among other molecules.
Bioactivity
Chemotaxis, degranulation.
Cell Type
Dendritic cells, Endothelial cells, Eosinophils, Monocytes, Neutrophils
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Schraufstatter IU, et al. 2009. J. Immunol. 182:3827.
2. Ippel JH, et al. 2009. J. Biol. Chem. 284:12363.
3. Monk PN, et al. 2007. Br. J. Pharmacol. 152:429.
4. Griffin RS, et al. 2007. J. Neurosci. 27:8699.
5. Lee H, et al. 2006. Nat. Biotechnol. 24:1279.
6. Buck E and Wells JA, et al. 2005. P. Natl. Acad. Sci. USA 102:2719.

Gene ID
12273 View all products for this Gene ID
UniProt
View information about CD88 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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