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PE anti-mouse CD96 (TACTILE) Antibody

Pricing & Availability
Clone
W20256B (See other available formats)
Regulatory Status
RUO
Other Names
Tactile (T cell-activated increased late expression)
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W20256B_PE_CD96_Antibody_1_100324-4
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε (clone 145-2C11) APC and anti-mouse CD96 (TACTILE) (clone W20256B) PE (left) or rat IgG2a, κ PE isotype control (right).
  • W20256B_PE_CD96_Antibody_1_100324-4
    C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε (clone 145-2C11) APC and anti-mouse CD96 (TACTILE) (clone W20256B) PE (left) or rat IgG2a, κ PE isotype control (right).
See PE spectral data
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166803 25 µg £94
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166804 100 µg £220
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Description

CD96, also known as TACTILE (T cell-activated increased late expression), is a type I transmembrane glycoprotein, belonging to the Ig superfamily. It is expressed on NK cells and T cells (upregulated after activation), but not on normal B cells and myeloid cells. CD96 is also found on some acute myeloid leukemia (AML) cells, and T cell acute lymphoblastic leukemia (T-ALL), as a leukemia stem cell-specific marker. A soluble form of CD96 has been found in serum. Human CD96, but not mouse CD96, is expressed as two isoforms due to alternative splicing. The main ligand of CD96 is CD155 (PVR), along with CD226 (DNAM-1) and TIGIT, and the binding is species-specific. Mouse CD96 has been reported to interact with nectin-1 (CD111), but human CD96 does not. CD96 may play an inhibitory role in immune responses. In CD96 deficient mice, NK cells produce more IFN-γ upon LPS treatment. In addition, CD96 negatively regulates NK cell function in mice, and blockade of CD96 with antibodies promotes anti-tumor responses and anti-metastatic activity in combination with anti-CTLA-4 or anti-PD-1. CD96 is considered to mediate activated T cell and NK cell adhesion to CD155-expressing target cells. CD96 also counterbalances CD226, which activates NK cell-mediated cytotoxicity via competing CD155 binding.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD96 (TACTILE)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone W20256B blocks the staining by clones 3.3 and QA19A37, while clones 3.3 and QA19A37 partially block the staining by clone W20256B.

Antigen Details

Structure
Type 1 single chain membrane glycoprotein of 569 amino acids. Member of the Ig superfamily with three Ig domains and extensive glycosylation.
Distribution

Positive on most peripheral T cells, NK cells and upregulated when activated

Function
CD96 is involved in NK cell adhesion and cytotoxicity via CD155.
Interaction
NK cells, NKT cells, T cells
Ligand/Receptor
CD155, nectin 1
Cell Type
NK cells, T cells
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Wang PL, et al. 1992. J Immunol. 148:2600-8.
  2. Seth S, et al. 2007. Biochem Biophys Res Commun. 364: 959-65.
  3. Blake SJ, et al. 2016. Clin Cancer Res. 22:5183-5188.
  4. Georgiev H, et al. 2018. Front Immunol. 9:1072.
  5. Mittal D, et al. 2019. Cancer Immunol Res. 7:559-571.
  6.  Chiang EY, et al. 2020. Eur J Immunol. 50: 891-902.

 

Gene ID
84544 View all products for this Gene ID
UniProt
View information about CD96 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 0    Revision Date: 01/01/0001

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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