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PE anti-mouse IL-33Rα (IL1RL1, ST2) Antibody

Pricing & Availability
Clone
DIH9 (See other available formats)
Regulatory Status
RUO
Other Names
T1/ST2, ST2L, IL-1R1, T1, DER4, IL1R1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
DIH9_PE_IL-33RaST2 _Antibody_FC_031813
Mouse Th2 clone D10.G4.1 was stained with anti-mouse IL-33Ra/ST2 (clone DIH9) PE (filled histogram) or rat IgG1, κ PE isotype control (open histogram).
  • DIH9_PE_IL-33RaST2 _Antibody_FC_031813
    Mouse Th2 clone D10.G4.1 was stained with anti-mouse IL-33Ra/ST2 (clone DIH9) PE (filled histogram) or rat IgG1, κ PE isotype control (open histogram).
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145303 25 µg £100
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145304 100 µg £234
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Description

IL-33Rα, also known as ST2 or IL-1RL1, is a member of the Toll/IL-1 receptor family.  It binds IL-33 and is structurally similar to IL-1R1.  Two forms of the protein exist - a soluble form known as ST2 and a membrane anchored form known as ST2L.  The membrane form is expressed by Th2 cells and bone marrow derived mast cells, whereas the soluble form is expressed by serum-stimulated fibroblasts.

Blocking with anti-ST2 antibodies has been shown to alleviate experimental arthritis and airway inflammation.  The IL-33-ST2 axis has been reported to be important across a range of diseases including asthma, allergies, and cardiac disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-33Rα-hFcγ1 fusion protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Hashiguchi M, et al. 2014. Eur. J. Immunology. (FC) PubMed
Product Citations
  1. Koenig A, et al. 2022. Front Immunol. 12:791100. PubMed
  2. Dragan M, et al. 2022. J Invest Dermatol. 142:583. PubMed
  3. Furuhashi K, et al. 2017. Immunology. 151:227. PubMed
  4. Karagiannis F, et al. 2020. Immunity. 52(4):620-634. PubMed
  5. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  6. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  7. Shani O, et al. 2020. Cancer Res. 80:5317. PubMed
  8. She L, et al. 2021. JCI Insight. 6:e143509. PubMed
  9. Wu G, et al. 2020. Br J Pharmacol. 177:5063. PubMed
  10. Wiesner D, et al. 2015. PLoS Pathog. 11:1004701. PubMed
  11. He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed
  12. Nakajima T, et al. 2021. J Exp Med. 218:. PubMed
  13. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  14. Klein O, et al. 2019. J Allergy Clin Immunol. 144:1074. PubMed
  15. Zhang M, et al. 2017. Mol Med Rep. 15:4291. PubMed
  16. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  17. Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed
  18. Dutton EE, et al. 2018. Wellcome Open Res. 2:117. PubMed
RRID
AB_2561914 (BioLegend Cat. No. 145303)
AB_2561914 (BioLegend Cat. No. 145304)

Antigen Details

Structure
63 kD, IL-1 receptor family member, single pass type I membrane protein
Distribution

Mast cells, Th2 cells, and fibroblasts

Function
Following interaction with IL-33, it recruits MyD88, Irak1, Irak4 and Traf6 followed by phosphorylation of the MAP/ERK pathway.
Interaction
Interacts with MyD88, Irak1, Irak4 and Traf6
Ligand/Receptor
IL-33
Cell Type
Fibroblasts, Mast cells, Th2
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokine/Chemokine Receptors
Antigen References

1. Yanagisawa K, et al. 1993. FEBS Lett. 318:83.
2. Schmitt E, et al. 1990. Cytokine 6:407.
3. Yanagisawa K, et al. 1992. FEBS Lett. 302:51.
4. Takagi T, et al. 1993. Biochim Biophys Acta. 1178:194.

Gene ID
17082 View all products for this Gene ID
UniProt
View information about IL-33Ralpha on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 02/18/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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