- Clone
- MK34 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Lymphocyte antigen 6K, Ly-6K, mLy-6K
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
151304 | 100 µg | £189 |
Lymphocyte antigen 6K (Ly6K) is a 17 kD GPI-anchored membrane protein expressed by testicular germ cells. Ly6K associates with TEX101 and may have a role in spermatogenesis. It is also expressed in some cancer cell types; a recent study also demonstrated its expression in plasma cells. A soluble 12 kD form of Ly6K has been reported.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse testicular homogenate.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissue sections1, western blotting1, and immunoprecipitation1.
-
Application References
(PubMed link indicates BioLegend citation) -
- Maruyama M, et al. 2010. Biochem. Biophys. Res. Commun. 402:75. (IHC-F, IP, WB)
- RRID
-
AB_2616815 (BioLegend Cat. No. 151304)
Antigen Details
- Structure
- GPI-anchored membrane protein of 17 kD, soluble protein of 12 kD.
- Distribution
-
Testicular germ cells, some cancer cell types, and plasma cells.
- Function
- Possible role in spermatogenesis.
- Interaction
- TEX101.
- Cell Type
- B cells, Plasma cells
- Biology Area
- Cancer Biomarkers, Cell Biology, Immunology
- Antigen References
-
1. Fujihara Y, et al. 2014. Biol. Reprod. 90:60.
2. von Allmen CE, et al. 2008. Mol. Immunol. 45:2727.
3. Yoshitake H, et al. 2008. Biochem. Biophys. Res. Commun. 372:277.
4. de Nooij-van Dalen AG, et al. 2003. Int. J. Cancer 103:768. - Gene ID
- 76486 View all products for this Gene ID
- UniProt
- View information about Ly6K on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All Ly6K Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse Ly6K | MK34 | FC,IHC-P,IHC-F,IP,WB |
PE anti-mouse Ly6K | MK34 | FC |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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