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PE anti-mouse TCR Vα2 Antibody

Pricing & Availability
Clone
B20.1 (See other available formats)
Regulatory Status
RUO
Other Names
T cell receptor Vα 2
Isotype
Rat IgG2a, λ
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Product Citations
publications
B20dot1_PE_030209.jpg
C57BL/6 splenocytes stained with 145-2C11 (CD3) APC and B20.1 PE
  • B20dot1_PE_030209.jpg
    C57BL/6 splenocytes stained with 145-2C11 (CD3) APC and B20.1 PE
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127807 25 µg £57
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127808 100 µg £130
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Description

The TCR alpha (α) chain complexes with the TCR beta (β) chain to form the T cell receptor in 95% of T cells, whereas the remaining 5% of T cells express gamma and delta chains (γ/δ).  TCR Vα2 is a distinct TCR subfamily found in mice having the a, b, and c haplotypes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Soluble TCR from mouse CTL clone KB5-C20
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The B20.1 antibody recognizes most members of the Va2 TCR subfamily in mice having the a, b, and c haplotypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Pircher H, et al. 1992. Eur. J. Immunol.. 22:399.
  2. Gregoire C, et al. 1991. P. Natl. Acad. Sci. USA 88:8077.
  3. Kao C, et al. 2005. Int. Immunol.17:1607. PubMed
  4. Steptoe RJ, et al. 2007. J. Immunol. 178:2094. PubMed
  5. Rao RR, et al. 2012. Immunity. 36:374. PubMed.
Product Citations
  1. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  2. Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed
  3. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  4. Alissafi T, et al. 2018. J Clin Invest. 128:3840. PubMed
  5. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  6. Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed
  7. Sartorius R, et al. 2015. EMBO Mol Med. 7: 973 - 988. PubMed
  8. Macdougall CE et al. 2018. Cell metabolism. 27(3):588-601 . PubMed
  9. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  10. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  11. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  12. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  13. Pizzolla A, et al. 2016. PLoS One. 11: 0160407. PubMed
  14. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  15. Bahmani B, et al. 2018. J Clin Invest. 128:4770. PubMed
  16. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
RRID
AB_1134183 (BioLegend Cat. No. 127807)
AB_1134183 (BioLegend Cat. No. 127808)

Antigen Details

Distribution

On a subset of T cells

Function
In combination of V β, Vα recognizes MHC/peptide complex on the antigen-presenting cells.
Interaction
Associate with Vβ and CD3 complex to form TCR complexes expressing on cell surface.
Ligand/Receptor
TCR recognizes MHC/peptide complex
Cell Type
T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Kubo RT, et al. 1989. J. Immunol.. 142:2736.
2. Pircher H, et al. 1992. Eur. J. Immunol. 22:399.

Gene ID
21473 View all products for this Gene ID
UniProt
View information about TCR Valpha2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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