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PE anti-mouse TCR Vβ8.1, 8.2 Antibody

Pricing & Availability
Clone
MR5-2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell receptor beta 8
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
MR5-2_PE_060710
BALB/c splenocytes stained with MR5-2 PE and CD3 APC
  • MR5-2_PE_060710
    BALB/c splenocytes stained with MR5-2 PE and CD3 APC
See PE spectral data
Cat # Size Price Quantity Check Availability Save
140103 25 µg £89
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140104 100 µg £155
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Description

Vβ8 T cell receptor (TCR Vβ8) subfamily has three members, Vβ8.1, 8.2, and 8.3. These subfamily members are variants of the TCR β chain that along with the TCR α chain forms the TCR heterodimer, responsible for the recognition of peptides presented by the MHC molecules. Murine Vβ8+ T cells recognize the superantigen staphylococcal enterotoxin B (SEB); in vivo administration of SEB results in the negative selection of Vβ8+ cells in the thymus. Vβ8 is one of the most frequently found β chains on the infiltrating T cells in the brain of mice with experimental autoimmune encephalomyelitis (EAE), and on the T cells response to Toxoplasma gondii.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
H-Y specific TH Clone OI6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: induction of proliferation of Vβ8.1+ and Vβ8.2+ T cells1,2 and immunohistochemical staining3.

Application References

(PubMed link indicates BioLegend citation)
  1. Kanagawa OJ. 1988. J. Immunol. Methods 110:169. (Activ)
  2. Denkers EY, et al. 1986. J. Immunol. 156:1089. (Activ)
  3. Drescher KM, et al. 2000. Int. Immunol. 12:271. (IHC)
  4. Huang JR, et al. 2014. J Immunol. 192:1972. PubMed
Product Citations
  1. Kuczynski EA, et al. 2022. EMBO Mol Med. 14:e15816. PubMed
  2. Fu X, et al. 2020. J Immunol. 205:2066. PubMed
  3. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  4. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  5. Lopes N, et al. 2022. Elife. 11:. PubMed
  6. Wang X, et al. 2022. Elife. 11:. PubMed
RRID
AB_10639942 (BioLegend Cat. No. 140103)
AB_10639942 (BioLegend Cat. No. 140104)

Antigen Details

Structure
Members of the immunoglobulin superfamily, Vβ8.1 and 8.2 are variants of the TCR β chain which associates with the TCR α chain to form the TCR heterodimer.
Distribution

Expressed in a subset of TCR αβ+ T cells

Function
Recognition of peptides presented in the MHC molecules, responsible for T cell mediated immune response
Ligand/Receptor
MHC-Peptide complex
Cell Type
T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Marrack P, et al. 2008. Annu. Rev. Immunol. 26:171.
2. Acha-Orbea H and MacDonald HR. 1995. Annu. Rev. Immunol. 13:459.
3. Sim GK and Augustin AA. 1985. Cell 42:89.
4. Mami-Chouaib F, et al. 2002. Immunol. Rev. 188:114.

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR Vbeta8.1 8.2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 02/05/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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