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PE anti-mouse TCR vβ8.3 Antibody

Pricing & Availability
Clone
1B3.3 (See other available formats)
Regulatory Status
RUO
Other Names
Tib, TCRB, TCRbeta
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
1B3dot3_PE_TCR-vbeta83_Antibody_022619
C57BL/6 mouse splenocytes were stained with CD3 APC and anti-mouse TCR vβ8.3 (clone 1B3.3) PE (left) or Armenian Hamster IgG Isotype control FITC (right).
  • 1B3dot3_PE_TCR-vbeta83_Antibody_022619
    C57BL/6 mouse splenocytes were stained with CD3 APC and anti-mouse TCR vβ8.3 (clone 1B3.3) PE (left) or Armenian Hamster IgG Isotype control FITC (right).
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156303 25 µg £89
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156304 100 µg £153
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Description

The TCR Vβ8 family consists of three members, namely Vβ8.1, 8.2 and 8.3. 1B3.3 reacts with Vβ8.3 only, but not Vβ8.1 or Vβ8.2. Most mouse strains have about 20% Vβ8+ T cells in the periphery. However, C57BR, C57L, SJL, SWR (Tcra haplotype) and RIII strains (Tcrc haplotype) do not express Vβ8 due to a gene deletion at the Vβ8 loci.  Exogenous superantigen, such as staphylococcal enterotoxin B, stimulates lymphocytes bearing Vβ8 and selectively eliminate those T cells in vivo.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
3.L2 T Cell Clone Expressing the vβ8.3 TCR
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone 1B3.3 recognizes TCR vß8.3 from both C57BL/6 and BALB/c strains.

Application References

(PubMed link indicates BioLegend citation)
  1. Kersh G, et al. 1998. J. Immunol. 161:585.
Product Citations
  1. Fu X, et al. 2020. J Immunol. 205:2066. PubMed
  2. Parthasarathy R, et al. 2021. Front Immunol. 12:758407. PubMed
  3. Wang Y, et al. 2022. Nat Cell Biol. 24:230. PubMed
RRID
AB_2800699 (BioLegend Cat. No. 156303)
AB_2800699 (BioLegend Cat. No. 156304)

Antigen Details

Structure
Ig super family; TCR is an 85-kd hetero-dimer composed of alpha and beta chain.
Distribution

Expressed in a subset of  αβ T cells

Function
Recognition of Peptide/MHC, T cell activation
Interaction
CD3
Ligand/Receptor
Peptide/MHC complex
Cell Targets
CD3
Cell Type
T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References
  1. Haskins K, et al. 1984. J. Exp. Med. 160:452
  2. Davis MM, et al. 1998. Annu Rev Immunol. 16:523
  3. Kersh GJ, et al. 1998. J Immunol. 161:585.
  4. Tomonari K. 1996. Immunogenetics. 44:73
Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR Vbeta8.3 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 02/12/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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