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PE anti-rat CD152 Antibody

Pricing & Availability
Clone
WKH203 (See other available formats)
Regulatory Status
RUO
Other Names
Cytotoxic T Lymphocyte-Associated Antigen-4 (CTLA-4), Ly-56
Isotype
Mouse IgG1, κ
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Product Citations
publications
WKH203_PE_1_082911
LOU rat lymph node cells were stimulated with plate-bound CD3 and CD28 for 3 days, then surface stained with CD4 PerCP. This was followed by fixation and permeabilization with BioLegend's FOXP3 Fix/Perm Buffer Set and intracellular staining with FOXP3 Alexa Fluor® 647, and CD152 (clone WKH203) PE (top) or mouse IgG1, κ PE isotype control (bottom). The data was analyzed by gating on CD4-positive cells.
  • WKH203_PE_1_082911
    LOU rat lymph node cells were stimulated with plate-bound CD3 and CD28 for 3 days, then surface stained with CD4 PerCP. This was followed by fixation and permeabilization with BioLegend's FOXP3 Fix/Perm Buffer Set and intracellular staining with FOXP3 Alexa Fluor® 647, and CD152 (clone WKH203) PE (top) or mouse IgG1, κ PE isotype control (bottom). The data was analyzed by gating on CD4-positive cells.
  • WKH203_PE_2_082911
See PE spectral data
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203007 25 µg £81
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Description

CD152 is a 33 kD member of the immunoglobulin superfamily also known as CTLA-4 or Ly-56. It is expressed on activated T and B lymphocytes. CD152 is similar to CD28 in amino acid sequence, structure, and genomic organization and these two receptors share common B7 family counter-receptors (B7-1, B7-2). Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses. CD152 is thought to play a role in the induction and maintenance of immunological tolerance, as well as the development of protective immunity and thymocyte regulation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Lin CH and Hunig T, et al. 2003. Eur. J. Immunol. 33:626. (IP)
  2. Nessi V, et al. 2010. J. Immunol. 185:5656. PubMed
RRID
AB_2276898 (BioLegend Cat. No. 203007)

Antigen Details

Structure
Ig superfamily, 33 kD
Distribution

Activated T and B cells

Function
Negative regulator of T cell activation
Ligand/Receptor
CD80 (B7-1), CD86 (B7-2)
Cell Type
B cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Allison JP, et al. 1995. Science 270:932.
3. Waterhouse P, et al. 1995. Science 270:985.
4. Linsley PS, et al. 1991. J. Exp. Med. 174:561.

Gene ID
63835 View all products for this Gene ID
UniProt
View information about CD152 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 02/14/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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