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PE anti-rat CD8a Antibody

Pricing & Availability
Clone
OX-8 (See other available formats)
Regulatory Status
RUO
Other Names
CD8a, T8, Leu-8, Ly-2, Lyt2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
OX-8_PE_020110
LOU rat splenocytes stained with OX-8 PE
  • OX-8_PE_020110
    LOU rat splenocytes stained with OX-8 PE
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
201705 50 µg £97
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201706 200 µg £184
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Description

CD8a is a 32 kD glycoprotein also known as T8, Lyt2, Ly-2, and CD8α. CD8a is a member of the immunoglobulin superfamily expressed on most thymocytes, subset of mature T cells, most NK cells, macrophages, and some activated CD4+ T cells (not resting). CD8a forms heterodimers with the CD8β chain (CD8b) on the surface of most thymocytes, while mature peripheral T lymphocytes express almost exclusively the CD8 αβ heterodimer. Intestinal intraepithelial lymphocytes express CD8a without CD8b. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
High molecular weight glycoproteins from rat thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The OX-8 antibody has been reported to partially block T cell responses, including mixed lymphocyte reactions and cytotoxic T cell responses, and to induce macrophage activation. Additional reported applications (for the relevant formats) include: immunohistochemistry1,2 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections, immunoprecipitation3, in vivo and in vitro blocking of T cell responses3, 4, macrophage stimulation5, and Western blotting3,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Barclay AN. 1981. Immunology 42:593. (IHC)
  2. Wallgren AC, et al. 1995. Transplantation 60:594. (IHC)
  3. Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841. (IP, WB)
  4. Mason DW, et al. 1983. Immunol. Rev. 74:57.
  5. Hirji N, et al. 1997. J. Immunol. 158:1833. (FA)
  6. Mitnacht R,, et al. 1998. J. Immunol. 160:700. (WB)
Product Citations
  1. Giles AJ, et al. 2019. JCI Insight. :4. PubMed
  2. Xie X, et al. 2018. FASEB J. 32:1296. PubMed
  3. Treacy O, et al. 2012. PLoS One. 7:e42662. PubMed
  4. Ni X, et al. 2019. Mol Med Rep. 19:3743. PubMed
RRID
AB_2075262 (BioLegend Cat. No. 201705)
AB_2075262 (BioLegend Cat. No. 201706)

Antigen Details

Structure
Ig superfamily, approximately 32 kD
Distribution

Thymocytes, NK cells, most CD8+ cells, intestinal intraepithelial lymphocytes, activated CD4+ cells, macrophages

Function
T cell activation, co-receptor for T cell activation
Ligand/Receptor
MHC class I molecule
Cell Type
Lymphocytes, Macrophages, NK cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Johnson P, et al. 1985. EMBO J. 4:2539.
2. Thomas ML, et al. 1983. Eur. J. Immunol. 13:855.

Gene ID
24930 View all products for this Gene ID
UniProt
View information about CD8alpha on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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