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PE anti-Syk Antibody

Pricing & Availability
Clone
5F5 (See other available formats)
Regulatory Status
RUO
Other Names
Spleen tyrosine kinase, protein tyrosine kinase SYK
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
5F5_PE_Syk_ICFC_1_021315
C57BL/6 mouse splenocytes were surface stained with CD19 Brilliant Violet 421™, then treated with the Nuclear Factor Fixation and Permeabilization Buffer Set, and intracellularly stained with anti-Syk (clone 5F5) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • 5F5_PE_Syk_ICFC_1_021315
    C57BL/6 mouse splenocytes were surface stained with CD19 Brilliant Violet 421™, then treated with the Nuclear Factor Fixation and Permeabilization Buffer Set, and intracellularly stained with anti-Syk (clone 5F5) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • 5F5_PE_Syk_ICFC_2_021315
See PE spectral data
Cat # Size Price Quantity Check Availability Save
646003 25 µg £105
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646004 100 µg £247
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Description

Syk is a cytoplasmic 72 kD non-receptor tyrosine kinase that is widely expressed in hematopoietic cells. The Syk kinase associates with a number of proteins including c-Src, Lck, Lyn, Fyn, Vav1, STAT3, SHP1, Grb2, and LAT to couple immunoreceptors to downstream signaling events mediating cellular proliferation, differentiation, and phagocytosis. Syk is modified by phosphorylation on multiple tyrosine sites.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse Syk peptide aa306-333
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.03 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.

Application References

(PubMed link indicates BioLegend citation)
  1. Chu D, et al. 1999. J. Immunol. 163:2610.
  2. Ackermann JA, et al. 2015. J Immunol. 194:4650. PubMed
Product Citations
  1. Leonardi I, et al. 2022. Cell. 185:831. PubMed
  2. Luo W et al. 2018. Immunity. 48(2):313-326 . PubMed
  3. Li X, et al. 2018. Cell Host Microbe. 24:847. PubMed
RRID
AB_2565305 (BioLegend Cat. No. 646003)
AB_2565305 (BioLegend Cat. No. 646004)

Antigen Details

Structure
Non-receptor associated tyrosine kinase, contains 2 SH2 domains, 72 kD
Distribution

Cytoplasmic protein, widely expressed in hematopoietic cells

Function
Couples immunoreceptor to downstream signaling events that mediate cellular responses including proliferation, differentiation, and phagocytosis
Biology Area
Cell Biology, Signal Transduction
Antigen References

1. Taniguchi T, et al. 1991. J. Biol. Chem. 266:15790.
2. Toyabe SL, et al. 2001.Immunology 103:164.

Gene ID
6850 View all products for this Gene ID
UniProt
View information about Syk on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 02/13/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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