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PE/Cyanine5 anti-mouse TIGIT (Vstm3) Antibody

Pricing & Availability
Clone
A17200C (See other available formats)
Regulatory Status
RUO
Other Names
T-Cell Immunoreceptor with Ig and ITIM domains, T cell Ig, Immunoglobulin Domain-Containing Protein 9, V-Set, Transmembrane Domain-Containing Protein 3, WUCAM
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A17200C_PE_Cyanine5_TIGIT_Antibody_041724
ConA-stimulated C57BL/6 mouse splenocytes (3 days) were stained with anti-mouse CD3ε (clone 145-2C11) APC and anti-mouse TIGIT (Vstm3) (clone A17200C) PE/Cyanine5 (left) or rat IgG2a, κ PE/Cyanine5 isotype control (right).
  • A17200C_PE_Cyanine5_TIGIT_Antibody_041724
    ConA-stimulated C57BL/6 mouse splenocytes (3 days) were stained with anti-mouse CD3ε (clone 145-2C11) APC and anti-mouse TIGIT (Vstm3) (clone A17200C) PE/Cyanine5 (left) or rat IgG2a, κ PE/Cyanine5 isotype control (right).
Compare all formats See PE/Cyanine5 spectral data
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622207 25 µg £110
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622208 100 µg £260
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Description

TIGIT (T cell immunoreceptor with Ig and ITIM domains precursor) contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif. TIGIT is an inhibitory receptor expressed on the surface of natural killer (NK) cells. TIGIT recognizes nectin and nectin-like adhesion molecules and thus plays a critical role in the innate immune response to malignant transformation. TIGIT in conjunction with CD226 forms a pathway that has prominent similarities to the costimulatory CD28/CTLA-4 pathway. CD226 and TIGIT bind the same set of ligands (CD155 and CD112) and CD226 is a positive regulator of T cell responses, while TIGIT inhibits them. Knockdown of TIGIT expression in human CD4+ T cells results in an increase of both T-bet and IFN-γ mRNA and protein expression. These effects are overcome by blocking CD226 signaling indicating that TIGIT exerts immunosuppressive effects by competing with CD226 for the same ligand, CD155. The binding of CD155 to TIGIT on human dendritic cells enhances the production of interleukin 10 and diminishes the production of interleukin 12p40. TIGIT shows an inhibitory effect on cell-signaling pathway in murine models of experimental autoimmune encephalomyelitis (EAE). Loss of TIGIT expression in mice results in hyperproliferative T cell responses and increased susceptibility to EAE.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse TIGIT Recombinant Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine5 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_3106166 (BioLegend Cat. No. 622207)
AB_3106166 (BioLegend Cat. No. 622208)

Antigen Details

Structure
Homodimer
Distribution

Human follicular B helper T cells (TFH), regulatory, memory and activated T cells, NK cells.

Function
Immunosuppressive effects by binding CD155 and modulating cytokine production by DC. Inhibits NK cell cytotoxicity. Inhibits T cell functions by competing with CD226. Tregs TIGIT+ cells suppress proinflammatory Th1 and Th17 cells, but not Th2 cell responses.
Interaction
Dendritic cells, monocytes
Ligand/Receptor
Poliovirus receptor (PVR/CD155) and nectin-2 (PRR-2/CD112)
Cell Type
NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family
Adhesion Molecules, Immune Checkpoint Receptors, Soluble Receptors
Antigen References
  1. Yu X, et al. 2009. Nat. Immunol. 10:48.
  2. Stanietsky N, et al. 2009. Proc. Natl. Acad. Sci. U S A. 106:17858.
  3. Joller N, et al. 2011. J. Immunol. 186:1338.
  4. Tahara-Hanaoka S, et al. 2004. Int. Immunol. 16:533.
  5. Lozano E, et al. 2012. J. Immunol. 188:3869.
  6. Joller N, et al. 2014. Immunity. 40:569.
  7. Deuss FA, et al. 2017. J. Biol. Chem. 292:11413.
Gene ID
100043314 View all products for this Gene ID
UniProt
View information about TIGIT on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/17/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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