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PE/Cyanine7 anti-human CD25 Antibody

Pricing & Availability
Clone
BC96 (See other available formats)
Regulatory Status
RUO
Workshop
V T-072
Other Names
Low affinity IL-2R, IL-2R α chain, Tac, p55
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
BC96_PECy7_071805
PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone BC96) PE/Cyanine7 (solid line) or mouse IgG1, κ PE/Cyanine7 isotype control (dotted line).
  • BC96_PECy7_071805
    PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone BC96) PE/Cyanine7 (solid line) or mouse IgG1, κ PE/Cyanine7 isotype control (dotted line).
Compare all formats See PE/Cyanine7 spectral data
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302611 25 tests £98
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302612 100 tests £200
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Description

CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications include: immunocytochemistry3.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  3. Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed
Product Citations
  1. Yuan H, et al. 2022. J Clin Exp Hematop. 62:52. PubMed
  2. Krammer S, et al. 2022. Cells. 12: . PubMed
  3. Vondra S, et al. 2023. Cell Rep. 42:111977. PubMed
  4. Veatch JR, et al. 2022. Cancer Cell. 40:393. PubMed
  5. Couturier J, et al. 2016. Retroviology. 13:30. PubMed
  6. Gkika E, et al. 2023. NPJ Precis Oncol. 7:24. PubMed
  7. Battistello E, et al. 2023. Mol Cell. 83:1216. PubMed
  8. Patskovsky Y, et al. 2023. Nat Commun. 14:3763. PubMed
  9. Rendeiro AF, et al. 2020. Nat Commun. 11:577. PubMed
  10. Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed
  11. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  12. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
  13. Massafra V, et al. 2021. J Immunol. 207:493. PubMed
  14. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  15. Palamides P, et al. 2016. Dis Model Mech. 9: 985 - 997. PubMed
  16. Kim ST, et al. 2022. Nat Commun. 13:1970. PubMed
  17. Wu W, et al. 2020. Oncol Lett. 0.91875. PubMed
  18. Huang B, et al. 2020. Cell. 179(5):1160-1176.e24.. PubMed
  19. Gorczynski RM, et al. 2017. Immunology. 150:418. PubMed
  20. Zirngibl F, et al. 2021. J Immunother Cancer. 9:. PubMed
  21. Acharya A, et al. 2020. J Virol. 95:e01657. PubMed
  22. Obradovic A, et al. 2021. Cell. 184(11):2988-3005.e16. PubMed
  23. Evans RDR, et al. 2020. Nat Commun. 3.491666667. PubMed
  24. Levring TB, et al. 2019. Sci Rep. 9:16725. PubMed
  25. Sayin I, et al. 2018. J Exp Med. 7:40286. PubMed
  26. Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed
  27. Xu X, et al. 2020. Arthritis Rheumatol. 72:1303. PubMed
  28. NULL, et al. 2022. Cell. 185:916. PubMed
  29. Weinberg A, et al. 2015. PLoS One. 10:122431. PubMed
  30. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  31. Groen B, et al. 2015. Sci Rep. 5: 13618. PubMed
  32. Gonzalez-Figueroa P, et al. 2021. Cell. 184(7):1775-1789.e19. PubMed
RRID
AB_314281 (BioLegend Cat. No. 302611)
AB_314281 (BioLegend Cat. No. 302612)

Antigen Details

Structure
Type I transmembrane glycoprotein, 55 kD
Distribution

Activated T cells and B cells, monocytes/macrophages, Treg

Function
Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
Ligand/Receptor
IL-2
Cell Type
B cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann T. 1991. J. Biol. Chem. 266:2681.

Gene ID
3559 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org
Go To Top Version: 3    Revision Date: 12/15/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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