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PE/Cyanine7 anti-human CD309 (VEGFR2) Antibody

Pricing & Availability
Clone
A16085H (See other available formats)
Regulatory Status
RUO
Other Names
VEGFR2, KDR
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A16085H_PEY7_CD309_Antibody_103018
HUVEC human endothelial cells were stained with CD309 (clone A16085H) PE/Cyanine7 (filled histogram) or mouse IgG1, κ isotype control (open histogram).
  • A16085H_PEY7_CD309_Antibody_103018
    HUVEC human endothelial cells were stained with CD309 (clone A16085H) PE/Cyanine7 (filled histogram) or mouse IgG1, κ isotype control (open histogram).
Compare all formats See PE/Cyanine7 spectral data
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393007 25 tests £121
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393008 100 tests £262
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Description

CD309, also known as VEGF-R2, KDR, and Flk-1 (mouse), is a type I transmembrane glycoprotein. It is a member of the CSF-1/PDGF receptor family of type III tyrosine kinase receptors. Human VEGF-R2 is mainly expressed by endothelial cells, embryonic tissues, and megakaryocytes. It plays an important role in the regulation of angiogenesis, vasculogenesis, and vascular permeability. The ligands of VEGF-R2 include VEGF-A, VEGF-C, and VEGF-D splice isoforms. Activation of VEGF-R2 with its ligands results in the receptor dimerization and autophosphorylation, stimulating endothelial cell proliferation and migration.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human VEGFR2-Fc Chimera
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This clone does not cross-react with mouse endothelial cells.

Product Citations
  1. Sparbier CE, et al. 2023. Nat Cell Biol. 25:258. PubMed
RRID
AB_2783297 (BioLegend Cat. No. 393007)
AB_2783297 (BioLegend Cat. No. 393008)

Antigen Details

Structure
Type I transmembrane glycoprotein, type III tyrosine kinase receptor, CSF-1/PDGF receptor family
Distribution

Endothelial cells, embryonic tissues

Function
VEGFR2 regulates angiogenesis as well as vascular development and permeability.
Ligand/Receptor
VEGF-A, VEGF-C, and VEGF-D splice isoforms
Cell Type
Endothelial cells
Biology Area
Angiogenesis, Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. DiSalvo J, et al. 1995. J Biol Chem. 270:7717.
  2. McColl BK, et al. 2003. J Exp Med. 198:863.
  3. Albuquerque RJ, et al. 2009. Nat Med. 15:1023.
  4. Guangqi E, et al. 2012. J Biol Chem. 287:3029.
  5. Koch S, and Claesson-Welsh. 2012. Cold Spring Harb Perspect Med. 2:a006502.
  6. Munaut C, et al. 2012. Plos One. 7:e33475.
  7. Wehland M, et al. 2013. Int J Mol Sci. 14:9338.
Gene ID
3791 View all products for this Gene ID
UniProt
View information about CD309 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/30/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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