Loading...

PE/Cyanine7 anti-mouse CD206 (MMR) Antibody

Pricing & Availability
Clone
C068C2 (See other available formats)
Regulatory Status
RUO
Other Names
MMR (macrophage mannose receptor), MR (mannose receptor), MRC1
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
C068C2_PECyanine7_CD206_Antibody_1_030724
Thioglycollate-elicited BALB/c mouse peritoneal macrophages were surface stained with CD107b (clone Mac-3) PE, and then intracellularly stained with CD206 (clone C068C2) PE/Cyanine7 (left) or rat IgG2a, κ PE/Cyanine7 isotype control (right).
  • C068C2_PECyanine7_CD206_Antibody_1_030724
    Thioglycollate-elicited BALB/c mouse peritoneal macrophages were surface stained with CD107b (clone Mac-3) PE, and then intracellularly stained with CD206 (clone C068C2) PE/Cyanine7 (left) or rat IgG2a, κ PE/Cyanine7 isotype control (right).
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
141719 25 µg £109
Check Availability


Need larger quantities of this item?
Request Bulk Quote
141720 100 µg £245
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD206, also known as mannose receptor (MR), is a 175 kD type I membrane protein. It is a pattern recognition receptor (PRR) belonging to the C-type lectin superfamily. MR is expressed on macrophages, dendritic cells, Langerhans cells, and hepatic or lymphatic endothelial cells. MR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine through its C-type lectin-like carbohydrate recognition domains, sulfated carbohydrate antigens through its cysteine-rich domain, and collagens through its fibronectin type II domain. MR mediates endocytosis and phagocytosis as well as activation of macrophages and antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity. Recently, MR on lymphatic endothelial cells was found to be involved in leukocyte trafficking and a contributor to the metastatic behavior of cancer cells. It suggests that MR may be a potential target in controlling inflammation and cancer metastasis by targeting the lymphatic vasculature.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD206 (MMR)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Keller J, et al. 2012. Biochem Biophys Res Commun. 417:217. PubMed
  2. Ito H, et al. 2012. J Am Soc Nephrol. 23:1797. PubMed
  3. Yang X, et al. 2015. PNAS. 112:2900. PubMed
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Rosina M, et al. 2022. Cell Metab. 34:533. PubMed
  2. Fang C, et al. 2022. J Pathol. 256:310. PubMed
  3. Xin B, et al. 2022. Hepatology. 76:630. PubMed
  4. Liang Y, et al. 2022. Theranostics. 12:7729. PubMed
  5. Yu J, et al. 2022. Nat Commun. 13:7903. PubMed
  6. Tunali G, et al. 2023. J Clin Invest. :. PubMed
  7. Sagnella SM, et al. 2020. Cancer Cell. 37(3):354-370. PubMed
  8. Solier S, et al. 2023. Int J Mol Sci. 24:. PubMed
  9. Liu N, et al. 2023. Cell Death Dis. 14:170. PubMed
  10. Schwarz N, et al. 2023. FASEB J. 37:e22846. PubMed
  11. Vanneste D, et al. 2023. Nat Immunol. 24:827. PubMed
  12. Chen Y, et al. 2023. J Nanobiotechnology. 21:98. PubMed
  13. He Q, et al. 2023. Parasit Vectors. 16:139. PubMed
  14. Zhang M, et al. 2020. Evid Based Complement Alternat Med. 8591892:2020. PubMed
  15. Accarias S, et al. 2020. J Cell Sci. 133:. PubMed
  16. Hu J, et al. 2021. Sci Adv. 7:. PubMed
  17. Mason HD, et al. 2021. JCI Insight. 6:e149229. PubMed
  18. Sepe JJ, et al. 2022. JACC Basic Transl Sci. 7:915. PubMed
  19. Zheng W, et al. 2021. Wellcome Open Research. 6:52. PubMed
  20. Zhou X, et al. 2022. Front Pharmacol. 13:887263. PubMed
  21. Ma C, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed
  22. Hreha TN, et al. 2020. Front Immunol. 1.597916667. PubMed
  23. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  24. Shen H, et al. 2022. Nat Commun. 13:6030. PubMed
  25. Brestoff JR, et al. 2021. Cell Metab. 33:270. PubMed
  26. Wu T, et al. 2021. J Exp Med. :218. PubMed
  27. Nagai Y, et al. 2019. Front Immunol. 10:174. PubMed
  28. Wu Q, et al. 2022. Cell Mol Life Sci. 79:137. PubMed
  29. Ling X, et al. 2021. ACS Nano. 15:765. PubMed
  30. Peng L, et al. 2020. Transl Lung Cancer Res. 0.892361111. PubMed
  31. Goggi JL, et al. 2020. Mol Imaging Biol. 22:1392. PubMed
  32. Zheng Y, et al. 2022. Front Pharmacol. 12:816032. PubMed
  33. Chen D, et al. 2020. Cancer Immunol Res. 8:883. PubMed
  34. Al Mamun A, et al. 2019. Neurochem Int. 127:148. PubMed
  35. Friedman DJ, et al. 2021. Cancer Immunol Res. 9:952. PubMed
  36. Kennedy EM, et al. 2022. Nat Commun. 13:5907. PubMed
  37. Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed
  38. Xiang W, et al. 2018. Nat Commun. 9:2574. PubMed
  39. Coronel MM, et al. 2020. Sci Adv. 6:eaba5573. PubMed
  40. Liu P, et al. 2022. iScience. 25:103867. PubMed
  41. Andriessen EMMA, et al. 2021. EMBO Mol Med. 13:e11754. PubMed
  42. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  43. Pizzurro GA, et al. 2021. Cancers (Basel). 13:. PubMed
  44. Wang G, et al. 2021. Cell Host Microbe. 29(5):777-791.e6. PubMed
  45. Nowak W, et al. 2020. EBioMedicine. 50:290-305.. PubMed
  46. Lam KC, et al. 2021. Cell. 184:5338. PubMed
  47. Upadhya R, et al. 2016. MBio. 7: 00547-16. PubMed
  48. Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed
  49. Chhatbar C et al. 2018. Cell reports. 25(1):118-129 . PubMed
  50. Weinstock NI, et al. 2020. Neuron. 107(1):65-81. PubMed
  51. Huang T, et al. 2022. Nat Commun. 13:3489. PubMed
  52. Panagi M, et al. 2020. Theranostics. 10:1910. PubMed
  53. Heinen A, et al. 2019. Mol Ther. 27:46:00. PubMed
  54. Ravussin A, et al. 2018. Cell Rep. 24:1085. PubMed
  55. Molgora M, et al. 2020. Cell. 182:886. PubMed
  56. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  57. Zhang L, et al. 2019. Front Immunol. 1.381944444. PubMed
  58. Piirainen S, et al. 2021. Glia. 69:2459. PubMed
  59. Xiao Z, et al. 2022. Mater Today Bio. 15:100297. PubMed
  60. Liu X, et al. 2021. Adv Sci (Weinh). 8:e2100233. PubMed
  61. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
  62. Scholz A, et al. 2016. EMBO Mol Med. 8: 39 - 57. PubMed
  63. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  64. Güç E, et al. 2020. Methods Enzymol. 91:632. PubMed
  65. Zhu Y et al. 2017. The Journal of Neuroscience. 37(9):2362-2376 . PubMed
  66. Zhang SJ, et al. 2021. Adv Sci (Weinh). 8:2003410. PubMed
RRID
AB_2562247 (BioLegend Cat. No. 141719)
AB_2562247 (BioLegend Cat. No. 141720)

Antigen Details

Structure
Type I transmembrane protein, 175 kD, C-type lectin superfamily
Distribution

Macrophages, dendritic cells, Langerhans cells, liver endothelial cells

Function
Pathogen recognition, endocytosis and phagocytosis, antigen presentation
Ligand/Receptor
Antigen containing mannose, fucose, or an N-acetyl glucosamine
Cell Type
Dendritic cells, Endothelial cells, Langerhans cells, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469.
3. Burgdorf S, et al. 2006. J. Immunol. 176:6770.
4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.

Gene ID
17533 View all products for this Gene ID
UniProt
View information about CD206 on UniProt.org

Related FAQs

Why is mouse CD206 stained intracellularly and not via surface staining?

Typically, mouse CD206 surface level is relatively low under normal conditions and so intracellular staining protocol is required to get better signal.

Go To Top Version: 2    Revision Date: 04/04/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account