PE/Cyanine7 anti-mouse CD28 Antibody anti-CD28

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Clone: E18 (See other available formats)
Regulatory Status: RUO
Other Names: Tp44, T44
Isotype: Mouse IgG2b, κ
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Product Citations: publications

E18_PECy7_020608 — C57BL/6 mouse thymocytes stained with E18 PE/Cyanine7

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122014

100 µg

£213

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Description

CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The E18 antibody blocks the binding of 37.51 antibody to CD28 and costimulates T cell activation and proliferation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: A20 cells and recombinant mCD28Ig
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: in vitro costimulation of T and NK cells¹, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity, and in vitro induction of thymocyte differentiation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 122004).
RRID: AB_604079 (BioLegend Cat. No. 122014)

Antigen Details

Structure: Ig superfamily, 44 kD
Distribution: Thymocytes, CD4⁺, CD8⁺ peripheral T cells, NK cells
Function: Costimulates T and NK cells
Ligand/Receptor: CD80 (B7-1), CD86 (B7-2)
Cell Type: NK cells, T cells, Thymocytes, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233.
3. Gross JA, et al. 1992. J. Immunol. 149:380.
Gene ID: 12487 View all products for this Gene ID
UniProt: View information about CD28 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

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Other Formats

View All CD28 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse CD28	E18	FC
FITC anti-mouse CD28	E18	FC
PE anti-mouse CD28	E18	FC
PE/Cyanine7 anti-mouse CD28	E18	FC
APC anti-mouse CD28	E18	FC
Alexa Fluor® 488 anti-mouse CD28	E18	FC
Ultra-LEAF™ Purified anti-mouse CD28	E18	FC,Block,Costim
Brilliant Violet 421™ anti-mouse CD28	E18	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

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