- Clone
- 3E12 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- NRP-1, VEGFR165 receptor
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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145211 | 25 µg | £121 | ||||
145212 | 100 µg | £262 |
CD304, also known as neuropilin-1, is a 140 kD type I transmembrane protein. Its extracellular region contains two CUB, two FV/FVIII, and one MAM domain. It is expressed by natural regulatory T cells (nTreg), a subset of invariant natural killer T cells (iNKT), endothelial cells, and neurons. Neuropilin-1 stabilizes the interaction between Tregs and dendritic cells, contributes to the recruitment of tumor-infiltrating Tregs in response to tumor-derived VEGF, and influences the process of angiogenesis and axon guidance. The ligands of CD304 are VEGF165 and semaphorin-3A.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Extracellular region of mouse CD304
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
-
Application References
(PubMed link indicates BioLegend citation) - Product Citations
-
- RRID
-
AB_2562359 (BioLegend Cat. No. 145211)
AB_2562359 (BioLegend Cat. No. 145212)
Antigen Details
- Structure
- Type I transmembrane protein, 140 kD, 2 complement binding domains (CUB), 2 coagulation factor V/VIII homology domains (FV/FVIII), one meprin, A5, receptor tyrosine phosphatase domain (MAM)
- Distribution
-
Natural regulatory T cells (nTreg), subset of invariant natural killer T cells (iNKT), endothelial cells, neurons
- Function
- Angiogenesis, axon guidance, modulation of the immune response
- Ligand/Receptor
- VEGF165, semaphorin-3A
- Cell Type
- Dendritic cells, Endothelial cells, Neurons, NKT cells, Tregs
- Biology Area
- Angiogenesis, Cancer Biomarkers, Cardiovascular Biology, Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
- Molecular Family
- Adaptor Proteins, Adhesion Molecules, CD Molecules
- Antigen References
-
1. Yadav M, et al. 2012. J. Exp. Med. 209:1713.
2. Weiss JM, et al. 2012. J. Exp. Med. 209:1723.
3. Hansen W, et al. 2012. J. Exp. Med. 209:2001.
4. Milpied P, et al. 2011. Blood 118:2993. - Gene ID
- 18186 View all products for this Gene ID
- UniProt
- View information about CD304 on UniProt.org
Related FAQs
Other Formats
View All CD304 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD304 (Neuropilin-1) | 3E12 | FC |
PE anti-mouse CD304 (Neuropilin-1) | 3E12 | FC |
APC anti-mouse CD304 (Neuropilin-1) | 3E12 | FC |
PerCP/Cyanine5.5 anti-mouse CD304 (Neuropilin-1) | 3E12 | FC |
Brilliant Violet 421™ anti-mouse CD304 (Neuropilin-1) | 3E12 | FC |
PE/Cyanine7 anti-mouse CD304 (Neuropilin-1) | 3E12 | FC |
Biotin anti-mouse CD304 (Neuropilin-1) | 3E12 | FC |
TotalSeq™-A0552 anti-mouse CD304 (Neuropilin-1) | 3E12 | PG |
PE/Dazzle™ 594 anti-mouse CD304 (Neuropilin-1) | 3E12 | FC |
TotalSeq™-B0552 anti-mouse CD304 (Neuropilin-1) | 3E12 | PG |
TotalSeq™-C0552 anti-mouse CD304 (Neuropilin-1) | 3E12 | PG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD304 (Neuropilin-1)
C57BL/6 mouse splenocytes were stained with purified CD304 (... -
PE anti-mouse CD304 (Neuropilin-1)
C57BL/6 mouse splenocytes were stained with CD4 APC and CD30... -
APC anti-mouse CD304 (Neuropilin-1)
C57BL/6 mouse splenocytes were stained with CD4 PE and CD304... -
PerCP/Cyanine5.5 anti-mouse CD304 (Neuropilin-1)
C57BL/6 mouse splenocytes were stained with CD4 FITC and CD3... -
Brilliant Violet 421™ anti-mouse CD304 (Neuropilin-1)
C57BL/6 mouse splenocytes were stained with CD4 APC and CD30... -
PE/Cyanine7 anti-mouse CD304 (Neuropilin-1)
C57BL/6 mouse splenocytes were stained with CD4 APC and CD30... -
Biotin anti-mouse CD304 (Neuropilin-1)
C57BL/6 mouse splenocytes were stained with CD4 APC and CD30... -
TotalSeq™-A0552 anti-mouse CD304 (Neuropilin-1)
-
PE/Dazzle™ 594 anti-mouse CD304 (Neuropilin-1)
C57BL/6 mouse splenocytes were stained with CD4 APC and CD30... -
TotalSeq™-B0552 anti-mouse CD304 (Neuropilin-1)
-
TotalSeq™-C0552 anti-mouse CD304 (Neuropilin-1)
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