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PE/Cyanine7 anti-mouse CD40 Antibody

Pricing & Availability
Clone
3/23 (See other available formats)
Regulatory Status
RUO
Other Names
Bp50, TNFRSF5
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
3slash23_PECy7_040111
BALB/c splenocytes stained with CD40 (clone 3/23) PE/Cyanine7 (filled histogram), or rat IgG2a, κ PE/Cyanine7 isotype control (open histogram).
  • 3slash23_PECy7_040111
    BALB/c splenocytes stained with CD40 (clone 3/23) PE/Cyanine7 (filled histogram), or rat IgG2a, κ PE/Cyanine7 isotype control (open histogram).
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
124621 25 µg £77
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124622 100 µg £238
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Description

CD40 is a 48 kD type I transmembrane glycoprotein also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD40 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 124627 &124628) with a low endotoxin limit (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Hasbold J, et al. 1994. Eur. J. Immunol. 24:1835.
  2. Bourgeois C, et al. 2002. Science 297:2060.
Product Citations
  1. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
  2. Zhu J, et al. 2022. Research (Wash D C). 2022:9816272. PubMed
  3. Pierini S, et al. 2021. J Immunother Cancer. 9:. PubMed
  4. Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
  5. Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
  6. Marangoni F, et al. 2021. Cell. . PubMed
  7. Huang YL, et al. 2015. Nat Commun. 6: 8141. PubMed
  8. Ciecko AE, et al. 2019. Cell Rep. 29:3073. PubMed
  9. Wu G, et al. 2020. Br J Pharmacol. 177:5063. PubMed
  10. Wang H, et al. 2019. Nat Commun. 10:1898. PubMed
  11. Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed
  12. Fontinha D, et al. 2015. PLoS One. 10: 0142540. PubMed
  13. Zhang J, et al. 2018. Sci Rep. 8:2373. PubMed
  14. Kramer K, et al. 2017. ACS Omega. 0.240972222. PubMed
  15. Elayeb R, et al. 2016. Haematologica. 101: 209 - 218. PubMed
  16. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  17. Lu X, et al. 2019. Circ Res. 125:1055. PubMed
  18. Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed
  19. Wu Y, et al. 2015. J Immunol. 195: 2612-2623. PubMed
  20. Silva-Cayetano A, et al. 2020. Med. 2(3):243-262.e8. PubMed
  21. Zhang X, et al. 2021. Front Pharmacol. 12:706748. PubMed
  22. Chen R, et al. 2021. Front Oncol. 11:743050. PubMed
  23. Wang Y, et al. 2020. Vaccines (Basel). 8:. PubMed
  24. Lal JC, et al. 2021. Breast Cancer Res. 23:83. PubMed
  25. Yang F, et al. 2021. Nat Commun. 12:3424. PubMed
RRID
AB_10897812 (BioLegend Cat. No. 124621)
AB_10897812 (BioLegend Cat. No. 124622)

Antigen Details

Structure
48 kD type I transmembrane glycoprotein, a member of the tumor necrosis factor receptor (TNFR) superfamily.
Distribution

Expressed on B lymphocytes and other antigen-presenting cells, such as macrophages, follicular dendritic cells, etc.

Function
Regulates B cell growth and differentiation and Ig isotype switching. Interaction of CD40 with its ligand, CD154, on activated T cells is important for immune response.
Ligand/Receptor
CD154 on T cells
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, Macrophages
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Grewal IS, et al. 1998. Annu Rev Immunol 16:111.

Gene ID
21939 View all products for this Gene ID
UniProt
View information about CD40 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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