PE/Dazzle 594 anti-human B7-H4 Antibody anti-B7-H4

Pricing & Availability

Clone: MIH43 (See other available formats)
Regulatory Status: RUO
Other Names: VTCN1, B7h.5
Isotype: Mouse IgG1, κ
Ave. Rating: Submit a Review
Product Citations: publications

MIH43_PEDZL594_B7-H4_Antibody_FC_092514 — Human B7-H4 transfected P815 cells were stained with B7-H4 (clone MIH43) PE/Dazzle™ 594 (filled histogram) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (open histogram).

Compare all formats See PE/Dazzle™ 594 spectral data

Cat #

Size

Price

Quantity Check Availability

Save

358112

100 tests

£262

Check Availability

Need larger quantities of this item?
Request Bulk Quote

Description

B7-H4, also known as VTCN1, is a type I transmembrane protein and member of the B7 family. Its extracellular region consists of one IgV-like and one IgC-like domain. B7-H4 expression has been reported on activated T cells, B cells, monocytes, and dendritic cells. On T cells, B7-H4 inhibits proliferation, cytokine secretion, and cytotoxicity. B7-H4 is also expressed by different carcinomas including renal, gastric, breast, ovarian and melanoma. Its expression is associated with a poor prognosis.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) includes immunofluorescence².
RRID: AB_2564337 (BioLegend Cat. No. 358112)

Antigen Details

Structure: Type I transmembrane protein, member of the B7 family, one IgV-like, one IgC-like domain
Distribution: Activated T cells, B cells, monocytes, and dendritic cells; different cancer types including melanoma, renal, gastric, breast and ovarian
Function: Inhibits T cell proliferation, cytokine secretion, and development of cytotoxicity
Cell Type: B cells, Dendritic cells, Monocytes, T cells, Tregs
Biology Area: Costimulatory Molecules, Immunology
Antigen References: 1. Fauci JM, et al. 2012. Gynecol. Oncol. 127:420.
2. Chen C, et al. 2012. J. Immunother. 35:354.
3. Guo G, et al. 2012. Clin. Rheumatol. 31:271.
4. Arigami T, et al. 2010. J. Surg. Oncol. 102:748.
Gene ID: 79679 View all products for this Gene ID
UniProt: View information about B7-H4 on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Related FAQs

There are no FAQs for this product.

Other Formats

View All B7-H4 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human B7-H4	MIH43	FC,IHC-F,ICC
PE anti-human B7-H4	MIH43	FC
PE/Cyanine7 anti-human B7-H4	MIH43	FC
APC anti-human B7-H4	MIH43	FC
PerCP/Cyanine5.5 anti-human B7-H4	MIH43	FC
PE/Dazzle™ 594 anti-human B7-H4	MIH43	FC
TotalSeq™-A0395 anti-human B7-H4	MIH43	PG
TotalSeq™-C0395 anti-human B7-H4	MIH43	PG
APC/Fire™ 750 anti-human B7-H4	MIH43	FC
TotalSeq™-B0395 anti-human B7-H4	MIH43	PG

Certificate of Analysis

Customers Also Purchased

Alexa Fluor® 700 anti-human CD155 (PVR)

Clone: SKII.4

Brilliant Violet 605™ anti-human HLA-A,B,C

Clone: W6/32

PerCP/Cyanine5.5 anti-human CD112 (Nectin-2)

Clone: TX31

Recombinant Human GM-CSF (carrier-free)

Go To Top Version: 2 Revision Date: 05/05/2015

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.