- Clone
- S17012B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Fcr-2, Fcr-3, Ly-17, LyM-1, Lym-1, fcRII, FcgRII, fcgr2b
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![S17012B_PEDazzle594_CD32_Antibody_030821 S17012B_PEDazzle594_CD32_Antibody_030821](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/S17012B_PEDazzle594_CD32_Antibody_030821.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with anti-mouse CD32 (clone S17012B) PE/Dazzle™ 594 (filled histogram) or rat IgG2b, ᴋ PE/Dazzle™ 594 isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
156411 | 25 µg | £97 | ||||
156412 | 100 µg | £238 |
CD32 (Fcgr2) is a 40 kD transmembrane glycoprotein, member of the immunoglobulin superfamily. The extracellular region of CD32 consists of two Ig C-type domains that binds the Fc region from monomeric IgG with low affinity, but binds immune complexes efficiently. CD32 can mediate phagocytosis of immune complexes and modulate cell activation. CD32 is expressed by Macrophages, neutrophils, mast cells and B cells.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse CD32 transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm. - Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- RRID
-
AB_2890717 (BioLegend Cat. No. 156411)
AB_2890717 (BioLegend Cat. No. 156412)
Antigen Details
- Structure
- 40 kD transmembrane glycoprotein, member of the immunoglobulin superfamily; extracellular region consists of two Ig C-type domains.
- Distribution
-
Macrophages, neutrophils, mast cells and B cells.
- Function
- Mediates phagocytosis of immune complexes, modulates cell activation.
- Ligand/Receptor
- Binds Fc from monomeric IgG with low affinity, binds immune complexes efficiently.
- Cell Type
- B cells, Macrophages, Mast cells, Neutrophils
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Fc Receptors
- Antigen References
-
- Negishi-Koga T, et al. 2015. Nat Commun. 6:6637
- Yamada DH, et al. 2015. Immunity. 42:379
- Clatworthy MR, et al. 2014. Nat Med. 20:1458
- Li F and Ravetch JV. 2011. Science. 333:1030
- Xiang Z, et al. 2007. Nat Immunol. 8:419
- Gene ID
- 14130 View all products for this Gene ID
- UniProt
- View information about CD32 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All CD32 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD32 (Fcgr2) | S17012B | FC |
PE anti-mouse CD32 (Fcgr2) | S17012B | FC |
APC anti-mouse CD32 (Fcgr2) | S17012B | FC |
FITC anti-mouse CD32 (Fcgr2) | S17012B | FC |
PE/Cyanine7 anti-mouse CD32 (Fcgr2) Antibody | S17012B | FC |
PE/Dazzle™ 594 anti-mouse CD32 (Fcgr2) Antibody | S17012B | FC |
Alexa Fluor® 647 anti-mouse CD32 (Fcgr2) | S17012B | FC |
Alexa Fluor® 700 anti-mouse CD32 (Fcgr2) | S17012B | FC |
PerCP/Cyanine5.5 anti-mouse CD32 (Fcgr2) | S17012B | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD32 (Fcgr2)
C57BL/6 mouse splenocytes were stained with CD19 APC and pur... Mouse CD32-transfected Ba/F3 cells were stained with purifie... Mouse CD16-transfected Ba/F3 cells were stained with purifie... -
PE anti-mouse CD32 (Fcgr2)
C57BL/6 splenocytes were stained with CD32 (clone S17012B) P... -
APC anti-mouse CD32 (Fcgr2)
C57BL/6 mouse splenocytes were stained with anti-mouse CD19 ... -
FITC anti-mouse CD32 (Fcgr2)
C57BL/6 mouse splenocytes were stained with anti-mouse CD19 ... -
PE/Cyanine7 anti-mouse CD32 (Fcgr2) Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse CD32 ... -
PE/Dazzle™ 594 anti-mouse CD32 (Fcgr2) Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse CD32 ... -
Alexa Fluor® 647 anti-mouse CD32 (Fcgr2)
C57BL/6 mouse splenocytes were stained with anti-mouse CD19 ... -
Alexa Fluor® 700 anti-mouse CD32 (Fcgr2)
C57BL/6 mouse splenocytes were stained with anti-mouse CD19 ... -
PerCP/Cyanine5.5 anti-mouse CD32 (Fcgr2)
C57BL/6 mouse splenocytes were stained with anti-mouse CD19 ...
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