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PE/Fire™ 640 Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Streptavidin- PE/Fire™, SAv- PE/Fire™ 640, streptavidin PE/Fire™ 640
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Product Citations
publications
Streptavidin_PE-Fire-640_Antibody_FC_101023
Human peripheral blood lymphocytes were stained with biotinylated anti-human CD3, followed by SAV- PE/Fire™ 640 (filled histogram), or biotinylated mouse IgG1, κ isotype control, followed with SAV- PE/Fire™ 640 (open histogram).
  • Streptavidin_PE-Fire-640_Antibody_FC_101023
    Human peripheral blood lymphocytes were stained with biotinylated anti-human CD3, followed by SAV- PE/Fire™ 640 (filled histogram), or biotinylated mouse IgG1, κ isotype control, followed with SAV- PE/Fire™ 640 (open histogram).
See PE/Fire™ 640 spectral data
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405375 25 µg £134
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405376 100 µg £291
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Description

Streptavidin is a 52.8 kDa tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used to detect biotinylated proteins in a wide range of applications.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
Streptavidin is conjugated with PE/Fire™ 640 under optimal conditions.
Concentration
0.2 mg/mL (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin PE/Fire™ 640 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the PE/Fire™ 640 conjugation. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire™ 640 has a maximum excitation of 566 nm and a maximum emission of 639 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Internal testing shows that PE Fire™/640 Streptavidin can interact non-specifically with human peripheral blood cells. To minimize non-specific binding of this tandem conjugate, use True-Stain Monocyte Blocker™.

Related FAQs

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Go To Top Version: 1    Revision Date: 10/10/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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