- Clone
- 12C2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- BDCA-4
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![12C2_PEFire810_CD304_Antibody_011323 12C2_PEFire810_CD304_Antibody_011323](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/12C2_PEFire810_CD304_Antibody_011323.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Human peripheral blood mononuclear cells were stained with anti-human CD303 FITC and anti-human CD304 (Neuropilin-1) (clone 12C2) PE/Fire™ 810 (left) or stained with anti-human CD303 FITC only. Data shown was gated on the lymphocyte and monocyte populations.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
354541 | 25 tests | £165 |
CD304, also known as neuropilin-1, BDCA-4 and VEGF165R, is a 140 kD type I transmembrane protein. Its extracellular region contains 2 CUB, 2 FV/FVIII, and one MAM domain; a soluble isoform is produced by alternative mRNA splicing. CD304 is involved in angiogenesis, neural development, and tumor metastasis. It's expressed by plasmacytoid dendritic cells, thymocytes, neurons, endothelium, and a subset of TFH cells. CD304 is also expressed in several carcinomas, and a high expression of this molecule in prostate cancer correlates with a poor prognosis.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- CD304-Fc Fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 810 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Fire™ 810 has a maximum excitation of 488/561 nm and a maximum emission of 810 nm.
Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect PE/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page. - Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- RRID
-
AB_2936538 (BioLegend Cat. No. 354541)
Antigen Details
- Structure
- Type I transmembrane protein, 140 kD, 2 complement binding domains (CUB), 2 coagulation factor V/VIII homology domains (FV/FVIII), one meprin, A5, receptor tyrosine phosphatase domain (MAM)
- Distribution
-
Plasmacytoid dendritic cells, thymocytes, subset of follicular helper T cells (TFH), endothelial cells, neurons, some carcinomas
- Function
- Angiogenesis, neuronal development, tumor metastasis
- Ligand/Receptor
- VEGF165, semaphorin-3A
- Cell Type
- Dendritic cells, Endothelial cells, Neurons, Tfh, Thymocytes
- Biology Area
- Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Mizui M and Kikutani H. 2008. Immunity 28:302.
2. Hamerlik P, et al. 2012. J. Exp. Med. 209:507.
3. Karjalainen K, et al. 2011. Blood 117:920.
4. Lepelletier Y, et al. 2007. P. Natl. Acad. Sci. USA 104:5545. - Gene ID
- 8829 View all products for this Gene ID
- UniProt
- View information about CD304 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All CD304 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human CD304 (Neuropilin-1)
-
PE anti-human CD304 (Neuropilin-1)
Human peripheral blood mononuclear cells were stained with C... -
APC anti-human CD304 (Neuropilin-1)
Human peripheral blood mononuclear cells were stained with C... -
PE/Cyanine7 anti-human CD304 (Neuropilin-1)
-
PerCP/Cyanine5.5 anti-human CD304 (Neuropilin-1)
Human peripheral blood mononuclear cells were stained with C... -
FITC anti-human CD304 (Neuropilin-1)
Human peripheral blood mononuclear cells were stained with C... -
Brilliant Violet 421™ anti-human CD304 (Neuropilin-1)
Human peripheral blood mononuclear cells were stained with C... -
Brilliant Violet 510™ anti-human CD304 (Neuropilin-1)
Human peripheral blood mononuclear cells were stained with C... -
Alexa Fluor® 647 anti-human CD304 (Neuropilin-1)
Human peripheral blood mononuclear cells were stained with C... -
Pacific Blue™ anti-human CD304 (Neuropilin-1)
Human peripheral blood mononuclear cells were stained with C... -
Biotin anti-human CD304 (Neuropilin-1)
Human peripheral blood mononuclear cells were stained with C... -
APC/Fire™ 750 anti-human CD304 (Neuropilin-1)
Human peripheral blood mononuclear cells were stained with C... -
TotalSeq™-A0406 anti-human CD304 (Neuropilin-1)
-
TotalSeq™-C0406 anti-human CD304 (Neuropilin-1)
-
TotalSeq™-B0406 anti-human CD304 (Neuropilin-1)
-
Brilliant Violet 605™ anti-human CD304 (Neuropilin-1)
Human peripheral blood mononuclear cells were stained with C... -
Brilliant Violet 711™ anti-human CD304 (Neuropilin-1)
Human peripheral blood mononuclear cells were stained with C... -
TotalSeq™-D0406 anti-human CD304 (Neuropilin-1)
-
PE/Dazzle™ 594 anti-human CD304 (Neuropilin-1)
Human peripheral blood mononuclear cells were stained with a... -
APC/Fire™ 810 anti-human CD304 (Neuropilin-1)
Human peripheral blood mononuclear cells were stained with a... -
PE/Fire™ 810 anti-human CD304 (Neuropilin-1)
Human peripheral blood mononuclear cells were stained with a... -
Brilliant Violet 785™ anti-human CD304 (Neuropilin-1)
Human peripheral blood mononuclear cells were stained with a... -
Brilliant Violet 650™ anti-human CD304 (Neuropilin-1)
Human peripheral blood mononuclear cells were stained with a... -
PE anti-human CD304
Typical results from human peripheral blood lymphocytes and ...
Follow Us