PE/Fire™ 810 anti-mouse Ly-6G Antibody, Ly-6G, 1A8

Pricing & Availability

Clone: 1A8 (See other available formats)
Regulatory Status: RUO
Other Names: Lymphocyte antigen 6 complex, locus G
Isotype: Rat IgG2a, κ
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Product Citations: publications

1A8_PEFire810_Ly-6G_Antibody_121421 — C57BL/6 mouse bone marrow cells were stained with anti-mouse CD11b APC and anti-mouse Ly-6G (clone 1A8) PE/Fire™ 810 (left) or CD11b APC only (right). Data shown were gated on the myeloid population.

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127673

25 µg

£117

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Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Ly-6G transfected EL-4J cell line.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 810 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire™ 810 has a maximum excitation of 488/561 nm and a maximum emission of 810 nm.

Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect PE/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C¹⁵. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8¹⁵. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4¹⁶.

Additional reported applications (for the relevant formats) include: immunohistochemistry⁹ of frozen sections¹⁰ and paraffin-embedded sections¹¹, depletion^{4, 12-14}, and spatial biology (IBEX)^20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).
RRID: AB_2910290 (BioLegend Cat. No. 127673)

Antigen Details

Structure: A 21-35 kD GPI-anchorded membrane protein
Distribution: Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.
Cell Type: Granulocytes, Macrophages, Monocytes
Biology Area: Immunology, Innate Immunity
Antigen References: Fleming TJ, et al. 1993. J. Immunol. 151:2399.
Gene ID: 546644 View all products for this Gene ID
UniProt: View information about Ly-6G on UniProt.org

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Other Formats

View All Ly-6G Reagents Request Custom Conjugation

Description	Clone	Applications
Alexa Fluor® 594 anti-mouse Ly-6G	1A8	IHC-F,SB
Purified anti-mouse Ly-6G	1A8	FC,IHC-F,IHC-P,SB
Biotin anti-mouse Ly-6G	1A8	FC,IHC
FITC anti-mouse Ly-6G	1A8	FC
PE anti-mouse Ly-6G	1A8	FC
Alexa Fluor® 647 anti-mouse Ly-6G	1A8	FC,IHC-F,SB
Pacific Blue™ anti-mouse Ly-6G	1A8	FC
APC anti-mouse Ly-6G	1A8	FC
PerCP/Cyanine5.5 anti-mouse Ly-6G	1A8	FC
PE/Cyanine7 anti-mouse Ly-6G	1A8	FC
Alexa Fluor® 700 anti-mouse Ly-6G	1A8	FC,IHC
APC/Cyanine7 anti-mouse Ly-6G	1A8	FC
Alexa Fluor® 488 anti-mouse Ly-6G	1A8	FC,IHC-F,SB
Brilliant Violet 421™ anti-mouse Ly-6G	1A8	FC,IHC-F
Brilliant Violet 570™ anti-mouse Ly-6G	1A8	FC
Ultra-LEAF™ Purified anti-mouse Ly-6G	1A8	FC,Depletion,IHC
Brilliant Violet 510™ anti-mouse Ly-6G	1A8	FC
Purified anti-mouse Ly-6G (Maxpar® Ready)	1A8	FC,CyTOF®,WB
Brilliant Violet 650™ anti-mouse Ly-6G	1A8	FC
Brilliant Violet 711™ anti-mouse Ly-6G	1A8	FC
Brilliant Violet 605™ anti-mouse Ly-6G	1A8	FC
Brilliant Violet 785™ anti-mouse Ly-6G	1A8	FC
PE/Dazzle™ 594 anti-mouse Ly-6G	1A8	FC
APC/Fire™ 750 anti-mouse Ly-6G	1A8	FC
PerCP anti-mouse Ly-6G	1A8	FC
TotalSeq™-A0015 anti-mouse Ly-6G	1A8	PG
TotalSeq™-C0015 anti-mouse Ly-6G	1A8	PG
TotalSeq™-B0015 anti-mouse Ly-6G	1A8	PG
Spark Blue™ 550 anti-mouse Ly-6G	1A8	FC
Spark NIR™ 685 anti-mouse Ly-6G	1A8	FC
Spark YG™ 593 anti-mouse Ly-6G	1A8	FC
APC/Fire™ 810 anti-mouse Ly-6G Antibody	1A8	FC
PE/Cyanine5 anti-mouse Ly-6G	1A8	FC
PE/Fire™ 810 anti-mouse Ly-6G Antibody	1A8	FC
Spark UV™ 387 anti-mouse Ly-6G	1A8	FC
PE/Fire™ 640 anti-mouse Ly-6G	1A8	FC
Spark YG™ 570 anti-mouse Ly-6G	1A8	IHC-F,FC
Spark Red™ 718 anti-mouse Ly-6G (Flexi-Fluor™)	1A8	FC
Spark Blue™ 574 anti-mouse Ly-6G (Flexi-Fluor™)	1A8	FC
PerCP/Fire™ 806 anti-mouse Ly-6G	1A8	FC

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Go To Top Version: 2 Revision Date: 05/03/2023

For Research Use Only. Not for diagnostic or therapeutic use.

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