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PE/Fire™ 810 anti-human CD223 (LAG-3) Antibody

Pricing & Availability
Clone
7H2C65 (See other available formats)
Regulatory Status
RUO
Other Names
CD223, LAG-3, LAG3, lymphocyte-activation gene-3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
7H2C65_PE-Fire-810_CD8_Antibody_092022
CD3/CD28/IL-2 stimulated (three days) peripheral blood monocular cells (PBMCs) were stained with anti-human CD8 Brilliant Violet 421™ and anti-human CD223 (clone 7H2C65) PE/Fire™ 810 (left) or stained with anti-human CD8 Brilliant Violet 421™ only (right).
  • 7H2C65_PE-Fire-810_CD8_Antibody_092022
    CD3/CD28/IL-2 stimulated (three days) peripheral blood monocular cells (PBMCs) were stained with anti-human CD8 Brilliant Violet 421™ and anti-human CD223 (clone 7H2C65) PE/Fire™ 810 (left) or stained with anti-human CD8 Brilliant Violet 421™ only (right).
Compare all formats See PE/Fire™ 810 spectral data
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369223 25 tests £121
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Description

CD223, also known as LAG-3, is a 70 kD type I transmembrane glycoprotein that is involved in T-cell signaling. Similar to CD4, CD223 binds MHC class II, but with a higher affinity. CD223 negatively regulates T-cell activation. It is expressed by activated T-cells and natural killer cells (NKs), as well as regulatory T-cells. It is transiently expressed on the surface of activated T-cells in acute conditions but high expression is maintained under tolerizing conditions. CD223 deficiency results in reduced tumor growth. CD223 and PD-1 can act in synergy and reverse exhausted phenotypes, improve tumor rejection, and control viral load.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human LAG-3 transfected cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 810 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire™ 810 has a maximum excitation of 488/561 nm and a maximum emission of 810 nm.


Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect PE/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The staining of clone 7H2C65 cannot be blocked by clone 11C3C65, which is another anti-human CD223 (LAG-3) antibody.

RRID
AB_2927867 (BioLegend Cat. No. 369223)

Antigen Details

Structure
70 kD transmembrane glycoprotein, Ig superfamily, highly homologous to CD4.
Distribution

Activated T-cells and natural killer cells (NKs) and regulatory T cells.

Function
Negatively regulates T-cell activation.
Ligand/Receptor
MHC class II molecules.
Cell Type
Dendritic cells, NK cells, T cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Castelli C, et al. 2014. Oncoimmunology 3(11):e967146.
2. Poirier N, et al. 2011. Clin. Exp. Immunol. 164:265.
3. Juno JA, et al. 2015. Retrovirology 12:17.
4. Casati C, et al. 2006. Cancer Res. 66:4450.

Gene ID
3902 View all products for this Gene ID
UniProt
View information about CD223 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/20/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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