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PE/Dazzle™ 594 anti-mouse CD314 (NKG2D) Antibody

Pricing & Availability
Clone
CX5 (See other available formats)
Regulatory Status
RUO
Other Names
CD314
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
CX5_PEDazzle594_CD314_Antibody_1_030718
C57/BL6 mouse splenocytes were stained with TruStain fcX™ (clone 93), then True-Stain Monocyte Blocker™ (Cat. No. 426103), anti-mouse CD49b (clone DX5) APC, and anti-mouse CD314 (clone CX5) PE/Dazzle™ 594 (left) or Rat IgG1, κ isotype control PE/Dazzle™ 594 (right).
  • CX5_PEDazzle594_CD314_Antibody_1_030718
    C57/BL6 mouse splenocytes were stained with TruStain fcX™ (clone 93), then True-Stain Monocyte Blocker™ (Cat. No. 426103), anti-mouse CD49b (clone DX5) APC, and anti-mouse CD314 (clone CX5) PE/Dazzle™ 594 (left) or Rat IgG1, κ isotype control PE/Dazzle™ 594 (right).
Compare all formats See PE/Dazzle™ 594 spectral data
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130213 25 µg £85
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130214 100 µg £230
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Description

This antibody has been reported to block binding of NKG2D to its ligands, RAE-1 and H60, and inhibition of NKG2D-dependent NK-cell cytotoxicity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse NKG2D
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

To reduce non-specific binding, preincubate cells with purified anti-mouse CD16/CD32, clone 93 (Cat No 101301/2) is recommended.

 

This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.

Application References

(PubMed link indicates BioLegend citation)
  1. Ogasawara K, et al. 2003. Immunity 18:41. (Block)
  2. Ogasawara K, et al. 2004. Immunity 20:757. (Block)
  3. Kong LY, et al. 2010. Clin. Cancer Res. 16:2550. PubMed
Product Citations
  1. Edwards SC, et al. 2023. J Exp Med. 220: . PubMed
  2. Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed
  3. Lu YJ, et al. 2021. Cell Rep. 36:109696. PubMed
  4. Kissiov DU, et al. 2022. Elife. 11:. PubMed
RRID
AB_2728147 (BioLegend Cat. No. 130213)
AB_2728147 (BioLegend Cat. No. 130214)

Antigen Details

Structure
NKG2D is a lectin-like type II transmembrane protein. It serves as a stimulatory receptor to activate NK cells via the non-covalently associated DAP10 or DAP 12 daptor protein.
Distribution

NK, NKT cells

Ligand/Receptor
RAE, H60,
Cell Type
NK cells, NKT cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Gene ID
27007 View all products for this Gene ID
UniProt
View information about CD314 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 03/09/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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