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PE/Dazzle™ 594 anti-mouse CD34 Antibody

Pricing & Availability
Clone
MEC14.7 (See other available formats)
Regulatory Status
RUO
Other Names
Mucosialin
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
MEC14-7_PEDazzle594_CD34_Antibody_022620
Mouse fibroblast cell line NIH/3T3 was stained with CD34 (clone MEC14.7) PE/Dazzle™ 594 (filled histogram) or rat IgG2a, κ PE/Dazzle™ 594 isotype control (open histogram).
  • MEC14-7_PEDazzle594_CD34_Antibody_022620
    Mouse fibroblast cell line NIH/3T3 was stained with CD34 (clone MEC14.7) PE/Dazzle™ 594 (filled histogram) or rat IgG2a, κ PE/Dazzle™ 594 isotype control (open histogram).
Compare all formats See PE/Dazzle™ 594 spectral data
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119329 25 µg £117
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119330 100 µg £257
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Description

CD34 is a highly glycosylated hematopoietic progenitor antigen. Two isoforms of CD34 have been reported to be generated by alternative splicing. This antigen is expressed on hematopoietic progenitors as well as on endothelial cells, brain, and testis. CD34 is thought to function as an adhesion molecule for early hematopoietic progenitors mediating the attachment of stem cells to extracellular matrix or stromal cells. CD34 is phosphorylated on serine residues by PKC.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Cells transfected with mouse CD34
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The MEC14.7 antibody does not stain bone marrow cells like some other mouse CD34 antibodies, probably because the antibody recognizes a different epitope from other mAbs. Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting6, and immunohistochemistry of acetone-fixed frozen sections and paraffin-embedded sections2,4,5,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Garlanda C, et al. 1997. Eur. J. Cell Biol. 73:368. (FC)
  2. Knowles HJ, et al. 2004. Circ. Res. 95:162. (IHC)
  3. Trempus CS, et al. 2003. J. Invest. Dermatol. 120:501.
  4. Winding B, et al. 2002. Clin. Cancer Res. 8:1932. (IHC)
  5. Voswinckel R, et al. 2003. Circ. Res. 93:372. (IHC)
  6. Kairaitis LK, et al. 2005. Am. J. Physiol. Renal. Physiol. 288:F198. (IHC, WB)
  7. Ao A, et al. 2008. P. Natl. Acad. Sci. USA 105:7821. PubMed
  8. Zaynagetdinov R., et al. 2011. J Immunol. 187:5703. PubMed.
RRID
AB_2832422 (BioLegend Cat. No. 119329)
AB_2832422 (BioLegend Cat. No. 119330)

Antigen Details

Structure
Type I membrane protein, 75-120 kD, highly glycosylated; two isoforms reported
Distribution

Hematopoietic progenitors, brain, testis, endothelial cells; low expression in thymus, spleen, and bone marrow

Function
Possible adhesion molecule thought to function in early hematopoiesis by mediating attachment of stem cells to bone marrow extracellular matrix or stromal cells. Presents carbohydrate ligands to selectins.
Ligand/Receptor
L-selectin, other selectins
Cell Type
Endothelial cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Garlanda C, et al. 1997. Eur. J. Cell Biol. 73:368.
2. Brown J, et al. 1991. Int. Immunol. 3:175.
3. Suda J, et al. 1992. Blood 79:2288.
4. Baumhueter S, et al. 1994. Blood 84:2554.

Gene ID
12490 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org
Go To Top Version: 1    Revision Date: 02/25/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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