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PerCP/Cyanine5.5 anti-human CD199 (CCR9) Antibody

Pricing & Availability
Clone
L053E8 (See other available formats)
Regulatory Status
RUO
Other Names
GPR 9-6, GPR28, CDw199, Chemokine Receptor 9
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
L053E8_PerCpCy5.5_CCR9_Antibody_FC_082013
Human acute lymphoblastic leukemia cell line, MOLT-4, was stained with CD199 (clone L053E8) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • L053E8_PerCpCy5.5_CCR9_Antibody_FC_082013
    Human acute lymphoblastic leukemia cell line, MOLT-4, was stained with CD199 (clone L053E8) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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358905 25 tests £121
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358906 100 tests £282
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Description

Human CD199, also known as CCR9, is a member of the G protein coupled receptor family and is involved in T cell development in the thymus and in the gut-associated immune response. It is highly expressed on different stages of thymocytes and upregulated on CD4+ CD8+ cells. Expression of CCR9 on γ/δ T cells in the intraepithelial and small intestine has been reported. The interaction of CCR9 with its ligand CCL25 (TECK, thymus-expressed chemokine) may direct the trafficking of developing T cells in the thymus and the generation of gut-specific immunological memory.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Cells transfected with human CCR9
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Product Citations
  1. Liu F, et al. 2016. PLoS Pathog. 12: 1005663. PubMed
  2. Hu H, et al. 2014. Proc Natl Acad Sci U S A. 111:13439. PubMed
  3. Mohns M, et al. 2015. J Virol. 89: 9748 - 9757. PubMed
RRID
AB_2562448 (BioLegend Cat. No. 358905)
AB_2562448 (BioLegend Cat. No. 358906)

Antigen Details

Structure
A member of G-protein coupled receptors which contains seven transmembrane domains. Human CCR9 has above 80% amino acid sequence homology with its mouse counterpart.
Distribution

CD4+CD8+ thymocytes, γ/δ T cells, subset of memory T cells

Function
May direct the trafficking of developing T cells in the thymus and generation of gut-specific immunological memory; co-receptor for HIV-1
Ligand/Receptor
CCL25
Cell Type
T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Zaballos A, et al. 1999. J. Immunol. 162:5671.
2. Wurbel MA, et al. 2007. J. Immunol. 178:7598.
3. Wurbel MA, et al. 2006. Eur. J. Immunol. 36:73.

Gene ID
10803 View all products for this Gene ID
UniProt
View information about CD199 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 08/21/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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