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PerCP/Cyanine5.5 anti-human CD205 (DEC-205) Antibody

Pricing & Availability
Clone
HD30 (See other available formats)
Regulatory Status
RUO
Other Names
DEC-205
Isotype
Mouse IgG1, κ
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Product Citations
publications
HD30_PerCPCyanine55_CD205_Antibody_101818
Monocyte-derived dendritic cells were stained with CD205 (DEC-205, clone HD30) PerCP/Cyanine5.5 (filled histogram), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • HD30_PerCPCyanine55_CD205_Antibody_101818
    Monocyte-derived dendritic cells were stained with CD205 (DEC-205, clone HD30) PerCP/Cyanine5.5 (filled histogram), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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342209 25 tests £141
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342210 100 tests £282
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Description

CD205 is a 210 kD C-type lectin transmembrane protein, known as DEC-205. It belongs to macrophage mannose receptor family and is found at high levels on dendritic cells and thymic epithelial cells. Unlike murine CD205, human CD205 is also expressed at low levels on T- and B-cells, NK cells and monocytes. CD205, serves as an endocytic receptor, functions in antigen uptake/processing and clearance of apoptotic cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a purified extracellular domain of human CD205-IgG1 Fc fusion protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of fixed, frozen sections4 and Western blotting4.

Application References

(PubMed link indicates BioLegend citation)
  1. Guo M, et al. 2000. Human Immunology 61:729.
  2. Cho H, et al. 2007. Physiol Genomics doi:10.1152/physiolgenomics.00051.2006
  3. Gurer C, et al. 2008. Blood 112:1231. PubMed
  4. Park CG, et al. 2012. J. Immunol. Methods 377:15. (IHC, WB)
RRID
AB_2137644 (BioLegend Cat. No. 342209)
AB_2137644 (BioLegend Cat. No. 342210)

Antigen Details

Structure
Macrophage mannose receptor family, C-type lectin, 210 kD
Distribution

High levels on dendritic cells and thymic epithelial cells, low levels on lymphocytes and monocytes

Function
Antigen uptake and process
Cell Type
Dendritic cells, Epithelial cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Guo M, et al. 2000. Human Immunology 61:729.
2. Kato M, et al. 2006. Int. Immunol.
3. Kato M, et al. 2003. J. Biol. Chem. 278(36):34035.
4. Small M and G. Kraal. 2003. Int. Immunol. 15(2):197.
5. Mahnke K, et al. 2000. J. Cell Biol. 151(3):673.

Gene ID
4065 View all products for this Gene ID
UniProt
View information about CD205 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 10/18/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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