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PerCP/Cyanine5.5 anti-human CD66b Antibody

Pricing & Availability
Clone
G10F5 (See other available formats)
Regulatory Status
RUO
Workshop
VI MA81
Other Names
CD67, CGM6, NCA-95, CEACAM8
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
G10F5_PerCPCyanine55_CD66b_Antibody_012919
Human peripheral blood granulocytes were stained with CD66b (clone G10F5) PerCP/Cyanine5.5 (filled histogram) mouse IgM, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • G10F5_PerCPCyanine55_CD66b_Antibody_012919
    Human peripheral blood granulocytes were stained with CD66b (clone G10F5) PerCP/Cyanine5.5 (filled histogram) mouse IgM, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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305107 25 tests £101
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305108 100 tests £238
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Description

CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen, formalin-fixed paraffin-embedded tissue sections, and spatial biology (IBEX)5,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  3. Norling LV, et al. 2012. Arterioscler Thromb Vasc Biol. 32:1970. PubMed
  4. Meinke P, et al. 2015. Neuroimmunol Discord. 25:127. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Fantone K, et al. 2021. Pathogens. 10:. PubMed
  2. Barnes CO, et al. 2022. Sci Adv. 8:eabp8155. PubMed
  3. Ismailova A, et al. 2023. Front Immunol. 14:1123344. PubMed
  4. Zheng Y, et al. 2022. Proc Natl Acad Sci U S A. 119:e2121077119. PubMed
  5. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  6. Thom S, et al. 2012. J Appl Physiol. 112:1268. PubMed
  7. Klichinsky M, et al. 2020. Nat Biotechnol. 38:947. PubMed
  8. Rodda LB, et al. 2020. Cell. 184(1):169-183.e17. PubMed
  9. Arcos J, et al. 2015. J Infect Dis. 212: 948-958. PubMed
  10. Xu J, et al. 2013. Toxicol Appl Pharmacol. 273:410. PubMed
  11. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  12. Szentkereszty M, et al. 2020. Pathol Oncol Res. 26:1117. PubMed
  13. Chu TY, et al. 2022. Nat Commun. 13:6385. PubMed
  14. Whiting C, et al. 2015. PLoS One. 10: 0133627. PubMed
  15. Hough KP, et al. 2020. Methods. 27:177. PubMed
  16. Kim MY, et al. 2018. Cell. 173:1439. PubMed
  17. Schoofs T et al. 2019. Immunity. 50(6):1513-1529 . PubMed
  18. Ram-Mohan N, et al. 2021. Life Sci Alliance. 4:. PubMed
  19. Yang M, et al. 2012. J Appl Physiol. 112:204. PubMed
  20. Hakki S, et al. 2022. Sci Rep. 12:1427. PubMed
  21. Wang Z, et al. 2020. J Cell Mol Med. . PubMed
  22. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  23. Metzemaekers M, et al. 2021. Clin Transl Immunology. 10:e1271. PubMed
  24. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  25. Tan EE, et al. 2020. J Clin Invest. 130:5817. PubMed
  26. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
  27. Barrera L, et al. 2017. Br J Cancer. 10.1038/bjc.2017.173. PubMed
  28. Pinkert J, et al. 2022. Oncoimmunology. 11:2008110. PubMed
  29. Thom S, et al. 2013. J Appl Physiol. 114:1396. PubMed
RRID
AB_2077856 (BioLegend Cat. No. 305107)
AB_2077856 (BioLegend Cat. No. 305108)

Antigen Details

Structure
Ig superfamily, CEA antigen group, GPI-linked glycoprotein, 95-100 kD
Distribution

Granulocytes

Function
Cell adhesion, neutrophil activation
Ligand/Receptor
CD66c
Cell Type
Granulocytes, Neutrophils
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kuijpers T, et al. 1993. J. Immunol. 151:4934.
2. Kuroki M, et al. 1992. J. Leuk. Biol. 52:551.

Gene ID
1088 View all products for this Gene ID
UniProt
View information about CD66b on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 01/29/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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