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PerCP/Cyanine5.5 anti-human CX3CR1 Antibody

Pricing & Availability
Clone
2A9-1 (See other available formats)
Regulatory Status
RUO
Other Names
V28, GPR13, Chemokine C-X3-C receptor 1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
2A9-1_PerCPCyanine55_CXCR1_Antibody_1_030424
Human peripheral blood lymphocytes were stained with CD3 FITC and CX3CR1 (clone 2A9-1) PerCP/Cyanine5.5 (left) or rat IgG2b, κ PerCP/Cyanine5.5 isotype control (right).
  • 2A9-1_PerCPCyanine55_CXCR1_Antibody_1_030424
    Human peripheral blood lymphocytes were stained with CD3 FITC and CX3CR1 (clone 2A9-1) PerCP/Cyanine5.5 (left) or rat IgG2b, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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341613 25 tests £113
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341614 100 tests £262
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Description

CX3CR1 is a G-protein-coupled seven-transmembrane chemokine receptor, also called GPR13 or V28. It is expressed on NK cells, T cell subset, monocytes/macrophages, dendritic cells, and some malignant epithelial cells. CX3CL1 (known also as fractalkine and neurotactin) is the ligand of CX3CR1. CX3CL1 is a unique transmembrane molecule with a CX3C-motif chemokine domain and a mucin-like stalk. CX3CL1 is expressed by activated-endothelial cells, neurons, and astrocytes. The interaction of CX3CR1 and its ligand mediatesfirm cell adhesion and migration.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CX3CR1-EGFP fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application References

(PubMed link indicates BioLegend citation)
  1. Nishimura M, et al. 2002. J. Immunol. 168:6173.
  2. Nanki T, et al. 2002. Arthritis Rheum. 46:2878.
  3. Kobayashi T, et al. 2007. Inflamm. Bowel Dis. 13:837.
  4. Beziat V, et al. 2011. J. Immunol. 186:6753. PubMed.
Product Citations
  1. Zhang M, et al. 2023. JCI Insight. 8: . PubMed
  2. Pachnio A, et al. 2016. PLoS Pathog. 12: 1005832. PubMed
  3. Akiyama H, et al. 2020. J Virol. . PubMed
  4. Krishnan S, et al. 2021. Clin Exp Immunol. 203:458. PubMed
  5. Castro-Amarante M, et al. 2016. J Virol. 90: 2195 - 2207. PubMed
  6. Krakow S, et al. 2019. Front Immunol. 10:1484. PubMed
RRID
AB_11219203 (BioLegend Cat. No. 341613)
AB_11219203 (BioLegend Cat. No. 341614)

Antigen Details

Structure
G-protein-coupled seven transmembrane receptor
Distribution

NK cells, T cell subset, monocytes/macrophages, dendritic cells

Function
cell adhesion and migration
Ligand/Receptor
CX3CL1 (Fractalkine, FKN, neurotactin)
Cell Type
Dendritic cells, Macrophages, Monocytes, NK cells, T cells
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Imai T, et al. 1997. Cell. 91:521.
2. Fong AM, et al. 1998. J. Exp. Med. 188:1413.
3. Auffray C, et al. 2009. J. Exp. Med. 206:595.

Gene ID
1524 View all products for this Gene ID
UniProt
View information about CX3CR1 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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