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PerCP/Cyanine5.5 anti-human GPR15 Antibody

Pricing & Availability
Clone
SA302A10 (See other available formats)
Regulatory Status
RUO
Other Names
BOB, Brother of Bonzo
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
SA302A10_-PerCP-Cyanine55_-GPR15_Antibody_060823
Human peripheral blood lymphocytes were stained with anti-human CD4 Brilliant Violet 421™ and anti-human GPR15 (clone SA302A10) PerCP/Cyanine5.5 (left) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
  • SA302A10_-PerCP-Cyanine55_-GPR15_Antibody_060823
    Human peripheral blood lymphocytes were stained with anti-human CD4 Brilliant Violet 421™ and anti-human GPR15 (clone SA302A10) PerCP/Cyanine5.5 (left) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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373015 25 tests £137
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373016 100 tests £310
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Description

GPR15, also known as BOB, is a G-protein coupled receptor with 7 transmembrane regions, that is present on the cell surface as an homotrimer. Its ligand, GPR15L, was recently identified. It acts as receptor for human immunodeficiency virus type 1 and 2. GPR15 is expressed by effector T cells and subsets of memory T cells, and is involved in the recruitment of proinflammatory CD4+ T cells responsible of the pathogenesis in colitis. It is upregulated on monocytes and neutrophils in the synovia of patients with RA.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human GPR15-transfected cells.
Formulation
PBS + NaN3 + 5% HPCD
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. 

Excitation Laser
Blue Laser (488 nm)
RRID
AB_3083362 (BioLegend Cat. No. 373015)
AB_3083362 (BioLegend Cat. No. 373016)

Antigen Details

Structure
G-protein coupled receptor with 7 transmembrane regions, present in the cell surface as homotrimer.
Distribution

Effector and memory T cells.

Cell Type
T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Nguyen LP, et al. 2015. Nat. Immunol. 16:207.
2. Kiene M, et al. 2014. PLoS One 9:e88195.
3. Cartwright A, et al. 2014. Cytokine. 67:53.
4. Kim SV, et al. 2013. Science. 340:1456.
5. Okamoto Y, et al. 2013. J. Biol. Chem. 288:9189. 

Gene ID
2838 View all products for this Gene ID
UniProt
View information about GPR15 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 06/08/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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