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PerCP/Cyanine5.5 anti-human IgG Fc Antibody

Pricing & Availability
Clone
M1310G05 (See other available formats)
Regulatory Status
RUO
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
M1310G05_PerCPCyanine55_IgG-Fc_Antibody_1_112123
Human peripheral blood lymphocytes were stained with CD19 FITC and IgG (clone M1310G05) PerCP/Cyanine5.5 (left) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
  • M1310G05_PerCPCyanine55_IgG-Fc_Antibody_1_112123
    Human peripheral blood lymphocytes were stained with CD19 FITC and IgG (clone M1310G05) PerCP/Cyanine5.5 (left) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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410709 25 tests £121
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410710 100 tests £262
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Description

IgG Fc is a homodimer that is composed of the constant region of the two heavy chains that form the IgG molecule. The Fc fragment mediates opsonization, antibody dependent cellular cytotoxicity (ADCC), and complement activation through binding to Fc receptors such as CD16, CD32, CD64, and the complement factor C1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human Siglec-E-IgG Fc fusion protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Clone M1310G05 recognizes IgG in the membrane of memory B cells, has a stronger affinity for IgG1 and IgG3 than for IgG2 and IgG4, and does not cross react with IgD, IgE, or IgM.

Product Citations
  1. Wildner NH, et al. 2021. J Leukoc Biol. 109:77. PubMed
  2. Sun P, et al. 2022. iScience. 25:105202. PubMed
  3. Cheng K, et al. 2023. Int J Mol Sci. 24:. PubMed
  4. Perik-Zavodskaia O, et al. 2023. Int J Mol Sci. :24. PubMed
  5. Tarke A, et al. 2022. Cell. 185:847. PubMed
  6. Tong P, et al. 2021. Cell. :. PubMed
  7. Meckiff BJ, et al. 2020. Cell. 183(5):1340-1353.e16. PubMed
  8. Long Y, et al. 2021. Biochem Biophys Res Commun. 571:152. PubMed
  9. Chen Y, et al. 2020. Cell. 1496:183. PubMed
RRID
AB_2565787 (BioLegend Cat. No. 410709)
AB_2565787 (BioLegend Cat. No. 410710)

Antigen Details

Structure
Homodimer formed by the constant region of the IgG heavy chain.
Ligand/Receptor
CD16, CD32, and CD64.
Cell Type
B cells
Biology Area
Immunology
Antigen References

1. Paul, WE. (2003). Fundamental Immunology. Philadelphia, PA: Lippincott, Williams, & Wilkins.

Gene ID
3500 View all products for this Gene ID
UniProt
View information about IgG Fc on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 05/21/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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