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PerCP/Cyanine5.5 anti-human/mouse SSEA-3 Antibody

Pricing & Availability
Clone
MC-631 (See other available formats)
Regulatory Status
RUO
Other Names
Stage-Specific Embryonic Antigen-3
Isotype
Rat IgM, κ
Ave. Rating
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Product Citations
publications
MC-631_PerCPCy55_SSEA-3_Antibody_022118
NCCIT (human teratocarcinoma cell line) were stained with anti-human/mouse SSEA-3 (clone MC-631) PerCP/Cyanine5.5 (filled histogram) or rat IgM, κ PerCP/Cyanine5.5 (open histogram).
  • MC-631_PerCPCy55_SSEA-3_Antibody_022118
    NCCIT (human teratocarcinoma cell line) were stained with anti-human/mouse SSEA-3 (clone MC-631) PerCP/Cyanine5.5 (filled histogram) or rat IgM, κ PerCP/Cyanine5.5 (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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330323 25 tests £113
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330324 100 tests £278
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Description

Stage-Specific Embryonic Antigen-3 (SSEA-3) is an internal core structure of glob-series glycolipids (R-Galβ1-3GalNAcβ1-3Galα1-4Galβ1-R). SSEA-3 is expressed on human teratocarcinoma cells (embryonal carcinoma, EC), embryonic stem cells (ESC), embryonic germ cells (EG), and preimplantation embryos. The expression of SSEA-3 on human ESC is downregulated upon differentiation. SSEA-3 is also expressed by mouse on unfertilized eggs and preimplantation to early cleaved embryos.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Reported Reactivity
African Green
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
4-8 cell stage mouse embryos
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1.

Application References

(PubMed link indicates BioLegend citation)
  1. Kannagi R, et al. 1983. EMBO 2:2355. (IF)
  2. Kaufmann B, et al. 2005. Virology 332:189. (ELISA)
Product Citations
  1. V ölkner C, et al. 2020. Stem Cell Research. 41:101606.. PubMed
RRID
AB_2728281 (BioLegend Cat. No. 330323)
AB_2728281 (BioLegend Cat. No. 330324)

Antigen Details

Distribution

Embryonic stemcells

Cell Type
Embryonic Stem Cells
Biology Area
Cell Biology, Immunology, Stem Cells
Gene ID
93961 View all products for this Gene ID
UniProt
View information about SSEA-3 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 02/26/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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