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PerCP/Cyanine5.5 anti-human TSLPR (TSLP-R) Antibody

Pricing & Availability
Clone
1B4 (See other available formats)
Regulatory Status
RUO
Other Names
Thymic stromal lymphopoietin protein receptor, cytokine receptor-like 2, CRL2, CRLF2, IL-XR, TSLP-R
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1B4_PerCPCyanine55_TSLPR_Antibody_082218
TSLPR transfected Ba/F3 cells were stained with TSLPR (Clone 1B4) PerCP/Cyanine5.5 (filled histogram), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • 1B4_PerCPCyanine55_TSLPR_Antibody_082218
    TSLPR transfected Ba/F3 cells were stained with TSLPR (Clone 1B4) PerCP/Cyanine5.5 (filled histogram), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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322812 100 tests £262
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Description

TSLP-R, also known as the thymic stromal lymphopoietin protein receptor, cytokine receptor-like 2, CRL2, and IL-XR, is a type I membrane receptor that forms a functional heterodimeric complex with IL-7R to bind with TSLP. The TSLP-R contains a WSXWS motif required for proper protein folding and a box1 motif important for association with the JAKs. TSLP-R has a predicted molecular weight approximately 40 kD, and has two isoforms reported that are produced by alternative splicing. The TSLP-R is expressed preferentially in myeloid cells, dendritic cells, and activated monocytes. It is weakly expressed in T cells. Expression has also been reported in heart, skeletal, muscle, and kidney tissues. TSLP binding to the heterodimeric functional receptor (TSLP-R and IL-7R) activates JAK2, STAT3, and STAT5 to stimulate cell proliferation. Ligand receptor interactions have been implicated in the development of the hematopoietic system, dendritic cell maturation, and the maintenance and polarization of human Th2 memory T cells in allergy diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human TSLP-R:Fc protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 322805/322806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG (Cat. No. 405303) second step, followed by SAv-PE (Cat. No. 405204)).

RRID
AB_2564355 (BioLegend Cat. No. 322812)

Antigen Details

Structure
Type I membrane receptor, forms functional heterodimeric complex with IL-7R to bind TSLP. Contains WSXWS motif required for proper protein folding and a box1 motif that is important for association with the JAKs.
Distribution

Expressed preferentially in myeloid cells including dendritic cells and activated monocytes, weakly expressed in T cells. Also expressed in heart, skeletal, muscle, and kidney cells.

Function
TSLP binding to functional receptor activates JAK2, STAT3, and STAT5 to stimulate cell proliferation. Has been implicated in the development of the hematopoietic system, dendritic cell maturation, and the maintenance and polarization of human Th2 memory T cells.
Interaction
Together with IL-7R binds TSLP
Cell Type
Dendritic cells, Monocytes, T cells, Tregs
Biology Area
Immunology
Molecular Family
Cytokine/Chemokine Receptors
Antigen References

1. Reche PA, et al. 2001. J. Immunol. 167:336.
2. Tonozuka Y, et al. 2001. Cytogenet. Cell. Genet. 93:23.
3. Zhang W, et al. 2001. Biochem. Biophys. Res. Commun. 281:878.
4. Wang YH, et al. 2006. Immunity 24:827.

Gene ID
64109 View all products for this Gene ID
UniProt
View information about TSLPR on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 08/22/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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