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PerCP/Cyanine5.5 anti-mouse CD21/CD35 (CR2/CR1) Antibody

Pricing & Availability
Clone
7E9 (See other available formats)
Regulatory Status
RUO
Other Names
CR2/CR1
Isotype
Rat IgG2a, κ
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Product Citations
publications
7E9_PerCPCy55_042909.jpg
C57BL/6 splenocytes stained with 7E9 PerCP/Cyanine5.5
  • 7E9_PerCPCy55_042909.jpg
    C57BL/6 splenocytes stained with 7E9 PerCP/Cyanine5.5
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
123415 25 µg £97
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123416 100 µg £238
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Description

CD21, also known as CR2 (complement receptor 2) and C3d receptor, binds C3d and iC3b. It is also a receptor of Epstein-Barr virus. CD35, also known as CR1, binds C3b, iC3b, C4b, and iC4b. CD21/CD35 is primarily expressed on B lymphocytes, mast cells, follicular dendritic cells, macrophages, and activated granulocytes. CD21/CD35 forms part of the B-cell antigen receptor complex with CD19 and CD81 and is involved in signal transduction.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CD35/CFA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application References

(PubMed link indicates BioLegend citation)
  1. Boackle S, et al. 2001 Immunity 15:775.
  2. de Andres B, et al. 2012. J. Immunol. 189:2300. PubMed
  3. Chiu YK, et al. 2014. J Immunol. 193:2207. PubMed
  4. Koening PA, et al. 2014. J Biol Chem. 289:34490. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Koenig A, et al. 2022. Front Immunol. 12:791100. PubMed
  2. Alexandre YO, et al. 2022. STAR Protoc. 3:101923. PubMed
  3. Gawish R, et al. 2022. Elife. 11:. PubMed
  4. Ercoli G, et al. 2021. Front Immunol. 11:611661. PubMed
  5. Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed
  6. Manzl C, et al. 2012. Cell Death Differ. 18:54. PubMed
  7. Na B, et al. 2016. PLoS One. 11: 0156429. PubMed
  8. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  9. Verheijen M, et al. 2020. Cell Rep. 108376:33. PubMed
  10. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  11. Steinel N, et al. 2014. J Immunol. 193:2881. PubMed
  12. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  13. Li D, et al. 2020. Immunohorizons. 0.661805556. PubMed
  14. Habener A, et al. 2021. J Allergy Clin Immunol. 147:2281. PubMed
RRID
AB_1595490 (BioLegend Cat. No. 123415)
AB_1595490 (BioLegend Cat. No. 123416)

Antigen Details

Structure
145 kD/190 kD type I transmembrane glycoprotein
Distribution

B cells, follicular dendritic cells

Function
Signal transduction
Ligand/Receptor
Associated with CD19/CD81. CD21 binds C3d, iC3b, and EBV; CD35 binds C3b, iC3b, C4b, and iC4b.
Cell Type
B cells, Dendritic cells
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kozono Y, et al. 1998. J. Immunol. 160:1562.
2. Shimizu I, et al. 2007. Blood 109:1773.
3. Roozendaal R and MC. Carroll. 2007. Immunol. Rev. 219:157.

Gene ID
12902 View all products for this Gene ID 12946 View all products for this Gene ID
UniProt
View information about CD21/CD35 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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