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PerCP/Cyanine5.5 anti-mouse CD45.1 Antibody

Pricing & Availability
Clone
A20 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5.1, LCA
Isotype
Mouse (A.SW) IgG2a, κ
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Product Citations
publications
A20_PerCPCy5dot5_011508
SJL mouse splenocytes stained with A20 PerCP/Cyanine5.5
  • A20_PerCPCy5dot5_011508
    SJL mouse splenocytes stained with A20 PerCP/Cyanine5.5
Compare all formats See PerCP/Cyanine5.5 spectral data
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110727 25 µg £81
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110728 100 µg £221
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Description

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  2. Brog RA, et al. 2022. Cancer Immunol Res. 10:962. PubMed
  3. Wang Y, et al. 2022. Cell Rep. 41:111738. PubMed
  4. Pleuger C, et al. 2022. Elife. 11: . PubMed
  5. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  6. Li X, et al. 2022. Cell. 185:1709. PubMed
  7. Reticker-Flynn NE, et al. 2022. Cell. 185:1924. PubMed
  8. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  9. Tas JMJ, et al. 2022. Immunity. 55:1856. PubMed
  10. Melzi E, et al. 2022. Immunity. 55:2168. PubMed
  11. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  12. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  13. Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed
  14. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  15. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  16. Sangesland M, et al. 2020. Immunity. 51(4):735-749. PubMed
  17. Liang Y, et al. 2020. J Immunol. 204:2984. PubMed
  18. Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed
  19. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  20. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  21. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  22. Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed
  23. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
  24. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  25. Beauchamp EM, et al. 2021. Blood Cancer Discov. 6:. PubMed
  26. Marangoni F, et al. 2021. Cell. . PubMed
  27. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  28. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  29. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  30. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  31. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  32. Kim C, et al. 2019. Cell Rep. 29:2202. PubMed
  33. Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed
  34. Quah B, et al. 2012. Cytometry A. 81:679. PubMed
  35. Sharma S, et al. 2021. Pathophysiology. 28:501. PubMed
  36. Lim JE, et al. 2022. Front Oncol. 12:1045016. PubMed
  37. Karimi M, et al. 2015. Blood. 125:3655. PubMed
  38. Platteel A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.044. PubMed
  39. Marcus A et al. 2018. Immunity. 49(4):754-763 . PubMed
  40. Krueger CC, et al. 2019. Front Immunol. 10:1831. PubMed
  41. Moutuou MM, et al. 2020. Methods Mol Biol. 2111:267. PubMed
  42. Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
  43. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  44. LM S, et al. 2016. Cell Rep. 16(12): 3286-96. PubMed
  45. Ma K, et al. 2022. iScience. 25:104347. PubMed
  46. Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
  47. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  48. Suan D et al. 2017. Immunity. 47(6):1142-1153 . PubMed
  49. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  50. Lamb T, et al. 2022. Toxics. 10:. PubMed
  51. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  52. Wang X, et al. 2021. EMBO J. 40:e105926. PubMed
  53. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  54. Lee SC et al. 2018. Cancer cell. 34(2):225-241 . PubMed
  55. X X, et al. 2016. J Immunol . 197: 1683-1691. PubMed
  56. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  57. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  58. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  59. Mujal AM, et al. 2022. Cancer Immunol Res. 10:403. PubMed
  60. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
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RRID
AB_893346 (BioLegend Cat. No. 110727)
AB_893346 (BioLegend Cat. No. 110728)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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