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PerCP/Cyanine5.5 anti-mouse CD79b (Igβ) Antibody

Pricing & Availability
Clone
HM79-12 (See other available formats)
Regulatory Status
RUO
Other Names
Ig β chain
Isotype
Armenian Hamster IgG
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Product Citations
publications
HM79-12_PerCPCyanine55_CD79b_Antibody_1_111723
C57BL/6 bone marrow cells stained with CD45R/B220 PE and CD79b (clone HM79-12) PerCP/Cyanine5.5 (left) or Armenian Hamster IgG isotype control PerCP/Cyanine5.5 (right).
  • HM79-12_PerCPCyanine55_CD79b_Antibody_1_111723
    C57BL/6 bone marrow cells stained with CD45R/B220 PE and CD79b (clone HM79-12) PerCP/Cyanine5.5 (left) or Armenian Hamster IgG isotype control PerCP/Cyanine5.5 (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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132809 25 µg £109
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132810 100 µg £245
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Description

Mouse CD79b (Igβ chain) is a 35-40kD transmembrane protein that forms a heterodimer with CD79a (30-35 kD, Ig α chain). The CD79b and CD79a hererodimers are associated with surface IgM to form the B-cell receptor (BCR) that is necessary for signal transduction via the BCR in mature B cells. CD79b participates in the signal transduction involved in development of B cells as well. It was reported that association between CD79b/CD79a with IgM is essential in inducing both the transition from progenitor to precursor B cells and subsequent allelic exclusion. Igβ knockout mice had a complete block in B cell development at the immature CD43+B220+ stage. The HM79b-12 clone reacts with an extracellular epitope of CD79b or Igβ.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.75 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application References

(PubMed link indicates BioLegend citation)
  1. Gong S, et al. 1996. Science. 272:411.
  2. Nagata K, et al. 1997. Immunity 7:559.
  3. Papavasiliou F, et al. 1995. 268:408.
Product Citations
  1. Jhingran A, et al. 2015. PLoS Pathog. e1004589:11. PubMed
  2. Huang A, et al. 2022. ACS Pharmacol Transl Sci. 1062:5. PubMed
  3. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
RRID
AB_2632917 (BioLegend Cat. No. 132809)
AB_2632917 (BioLegend Cat. No. 132810)

Antigen Details

Structure
A 35-40 kD transmembrane protein which forms an 80-90 kD heterodimer with CD79a.
Distribution

CD79b expression is restricted to pro-B cell, pre-B cell, and mature B cells.

Ligand/Receptor
CD79a/CD79b heterodimers are associated with surface IgM to form B cell receptor complex (BCR) to recognize specific antigen.
Cell Type
B cells
Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
15985 View all products for this Gene ID
UniProt
View information about CD79b on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 12/22/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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