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PerCP/Cyanine5.5 anti-mouse CD86 Antibody

Pricing & Availability
Clone
GL-1 (See other available formats)
Regulatory Status
RUO
Other Names
B7-2, B70, Ly-58
Isotype
Rat IgG2a, κ
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Product Citations
publications
GL-1_PerCPCyanine55_CD86_Antibody_081618
LPS-stimulated (3 days) C57BL/6 mouse splenocytes were stained with CD86 (Clone GL-1) PerCP/Cyanine5.5 (filled histogram), or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • GL-1_PerCPCyanine55_CD86_Antibody_081618
    LPS-stimulated (3 days) C57BL/6 mouse splenocytes were stained with CD86 (Clone GL-1) PerCP/Cyanine5.5 (filled histogram), or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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105027 25 µg £81
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105028 100 µg £221
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Description

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
LPS-activated CBA/Ca mouse splenic B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The GL-1 antibody can block the mixed lymphocyte reaction in vitro and has been shown to inhibit the priming of cytotoxic T lymphocytes in vivo (along with antibodies against B7-1). Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections2,6, immunofluorescence microscopy, and in vivo and in vitro blocking of T cell responses1-6. GL-1 is not suitable for immunohistochemical staining of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105051-105056).

Application References

(PubMed link indicates BioLegend citation)
  1. Hathcock KS, et al. 1993. Science 262:905. (Block, IP)
  2. Inaba KM, et al. 1994. J. Exp. Med. 180:1849. (Block, IHC)
  3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
  4. Krummel MF, et al. 1995. J. Exp. Med. 182:459. (Block)
  5. Liu Y, et al. 1997. J. Exp. Med. 185:251. (Block)
  6. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  9. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
  10. Klinger MB, et al. 2007. Am. J. Physiol. Requl. Integr. Comp. Physiol. 293:R677. PubMed
  11. de Verteuil DA, et al. 2014. J Immunol. 193:1121. PubMed
Product Citations
  1. Kawabe T, et al. 2020. Nat Commun. 2.795833333. PubMed
  2. Wang S, et al. 2022. Sci Adv. 8:eabn3883. PubMed
  3. Campisi L, et al. 2022. Nature. 606:945. PubMed
  4. Liu H, et al. 2023. Cell Death Differ. :. PubMed
  5. Adzika GK, et al. 2023. Front Immunol. 14:1124649. PubMed
  6. Fu S, et al. 2023. Nat Commun. 14:2248. PubMed
  7. Bhaskar A, et al. 2023. iScience. 26:106644. PubMed
  8. Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed
  9. Ortiz G, et al. 2020. Front Immunol. 11:1713. PubMed
  10. Miller CM, et al. 2020. J Virol. 94:00:00. PubMed
  11. Szulc-D&acedil;browska L, et al. 2017. PLoS One. 12(6):e0179166. PubMed
  12. Zhu J, et al. 2016. Sci Rep. 6:27136. PubMed
  13. Franks SE, et al. 2019. J Immunol. 202:3381. PubMed
  14. Hou H, et al. 2021. Front Cell Dev Biol. 9:737003. PubMed
  15. Wang H, et al. 2019. Nat Commun. 10:1898. PubMed
  16. Ghilas S, et al. 2021. iScience. 24:103059. PubMed
  17. Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed
  18. Ribeiro-Gomes F, et al. 2015. Cell Death Dis. 6: 2018. PubMed
  19. Ise W, et al. 2018. Immunity. 48:702. PubMed
  20. Tripathi H, et al. 2020. Stem Cell Rev Rep. 0.953472222. PubMed
  21. Fusciello M, et al. 2022. Mol Ther Oncolytics. 25:137. PubMed
  22. Nikolos F, et al. 2022. Nat Commun. 13:1487. PubMed
  23. Puigdelloses M, et al. 2021. J Immunother Cancer. 9:. PubMed
  24. Rashid MH, et al. 2021. Oncol Rep. 45:1171. PubMed
  25. Ali S, et al. 2021. PLoS One. 16:e0246646. PubMed
  26. Yang X, et al. 2022. Front Immunol. 13:856230. PubMed
  27. Wang G, et al. 2021. Cell Host Microbe. 29(5):777-791.e6. PubMed
  28. Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed
  29. Hammer A, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01922. PubMed
  30. Niven J, et al. 2019. Cell Rep. 28:21. PubMed
  31. Raso F, et al. 2018. J Clin Invest. 128:4163. PubMed
  32. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  33. Siolas D, et al. 2021. Cell Reports. 36:109578. PubMed
  34. Denieffe S, et al. 2013. Brain Behav Immun. 34:86. PubMed
  35. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  36. Lal JC, et al. 2021. Breast Cancer Res. 23:83. PubMed
  37. Sadiq BA, et al. 2020. Current Protocols in Immunology. 131(1):e115. PubMed
  38. Hayashi K, et al. 2020. Nat Commun. 4.832638889. PubMed
RRID
AB_2074994 (BioLegend Cat. No. 105027)
AB_2074994 (BioLegend Cat. No. 105028)

Antigen Details

Structure
Ig superfamily, 80 kD
Distribution

B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes

Function
T cell costimulation, Ig class-switching, NK cell cytotoxicity
Ligand/Receptor
CD28, CD152 (CTLA-4)
Cell Type
Astrocytes, B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Hathcock KS, et al. 1993. Science 262:905.
3. Freeman GJ, et al. 1993. Science 262:907.
4. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.

Gene ID
12524 View all products for this Gene ID
UniProt
View information about CD86 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 08/17/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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