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PerCP/Cyanine5.5 anti-mouse CD8b Antibody

Pricing & Availability
Clone
YTS156.7.7 (See other available formats)
Regulatory Status
RUO
Other Names
Lyt-3, Ly-3
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
YTS15677_PerCPCyanine55_CD8b_Antibody_083118
C57BL/6 mouse splenocytes were stained with CD3e APC and CD8b (Clone YTS156.7.7) PerCP/Cyanine5.5 (left), or rat IgG2b, κ isotype control PerCP/Cyanine5.5 (right).
  • YTS15677_PerCPCyanine55_CD8b_Antibody_083118
    C57BL/6 mouse splenocytes were stained with CD3e APC and CD8b (Clone YTS156.7.7) PerCP/Cyanine5.5 (left), or rat IgG2b, κ isotype control PerCP/Cyanine5.5 (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
126609 25 µg £81
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126610 100 µg £221
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Description

CD8b is the 32 kD β chain of CD8, also known as Lyt-3 or Ly-3. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. McNical AM, et al. 2007. Eur. J. Immunol. 37:1634.
Product Citations
  1. Lucca L, et al. 2014. J Immunol. 193:3267. PubMed
  2. Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed
  3. Fiege JK, et al. 2021. Cell Host Microbe. 29:1815. PubMed
  4. Zhang W, et al. 2022. Dev Cell. 57:329. PubMed
  5. Konjar S, et al. 2022. Proc Natl Acad Sci U S A. 119:e2202144119. PubMed
  6. Mackin S, et al. 2023. Nat Microbiol. 8:569. PubMed
  7. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  8. Acker A, et al. 2014. PLoS One. 9:110015. PubMed
  9. Gimblet C et al. 2017. Cell host & microbe. 22(1):13-24 . PubMed
  10. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  11. Zhu B, et al. 2021. Immunity. 54(6):1200-1218.e9. PubMed
  12. Jin RM, et al. 2017. J Immunol. 198:352. PubMed
  13. Hossain DMS, et al. 2018. J Clin Invest. 128:644. PubMed
  14. Hum NR, et al. 2022. Front Oncol. 12:928474. PubMed
  15. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
  16. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  17. Hassan AO, et al. 2020. Cell. 183:169. PubMed
  18. de Reuver R, et al. 2021. Cell Reports. 36(6):109500. PubMed
  19. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  20. Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed
  21. Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed
  22. Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed
  23. Bennion BG, et al. 2019. J Virol. 93. PubMed
  24. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  25. Sivaram N, et al. 2019. J Clin Invest. 130. PubMed
  26. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  27. Wang Y, et al. 2022. Nat Cell Biol. 24:230. PubMed
RRID
AB_2260149 (BioLegend Cat. No. 126609)
AB_2260149 (BioLegend Cat. No. 126610)

Antigen Details

Structure
Ig superfamily, CD8β chain, 34 kD
Distribution

Majority of thymocytes, cytotoxic T cell subsets

Function
Co-receptor of TCR
Ligand/Receptor
MHC class I
Cell Type
T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1.Barclay A, et al. 1997. The Leukocyte antigen Facts Book Academic Press.
2. Zamoyska R. 1994. Immunity 1:243-246.
3. Ellmeier W, et al. 1999. Annu. Rev Immunol 17:523.
4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503.

Gene ID
12526 View all products for this Gene ID
UniProt
View information about CD8beta on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 08/31/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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