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PerCP/Cyanine5.5 anti-mouse Granzyme C Antibody

Pricing & Availability
Clone
SFC1D8 (See other available formats)
Regulatory Status
RUO
Other Names
CCP2, Ctla5, Ctla-5, AI323531, B10, GzmC
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
SFC1D8_PerCPCyanine55_GranzymeC_Antibody_113023
CD3/CD28 stimulated BALB/c splenocytes (in the presence of recombinant mouse IL-2) were stained with CD8 APC and Granzyme C (clone SFC1D8) PerCP/Cyanine5.5 (left) or Armenian Hamster IgG PerCP/Cyanine5.5 isotype control (right).
  • SFC1D8_PerCPCyanine55_GranzymeC_Antibody_113023
    CD3/CD28 stimulated BALB/c splenocytes (in the presence of recombinant mouse IL-2) were stained with CD8 APC and Granzyme C (clone SFC1D8) PerCP/Cyanine5.5 (left) or Armenian Hamster IgG PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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150809 25 µg £121
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150810 100 µg £262
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Description

Granzyme C is a serine protease that is found in the granules of cytotoxic T lymphocytes and natural killer cells (NKs). It induces the apoptosis of target cells by the externalization of phosphatidylserine, nuclear condensation and collapse, as well as the introduction of single-stranded DNA nicks. It can also induce apoptosis via mitochondrial swelling, which results in the loss of membrane potential. Reported studies show that granzyme C can support CTL-mediated killing in the absence of granzyme A or B. Additionally, wild-type mice have delayed upregulation of granzyme C relative to granzyme B and a mutation in the granzyme B gene shows earlier upregulation of granzyme C. Further studies show that in the event that granzyme B fails, the mouse models infected with persistent viruses have delayed expression of protection provided by granzyme C.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Purified recombinant granzyme C in CFA and boosted in IFA.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Cai Sheng, et al. 2009. J. Immunol. 182:6287. (ICFC)
RRID
AB_2734216 (BioLegend Cat. No. 150809)
AB_2734216 (BioLegend Cat. No. 150810)

Antigen Details

Distribution

Cytotoxic T lymphocytes and natural killer (NK) cells.

Function
Induces apoptotic cell death.
Cell Type
NK cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Enzymes and Regulators, Proteases
Antigen References

1. Bots M, et al. 2006. J. Cell. Sci. 119:5011.
2. Ley TJ, et al. 2003. Blood 101:3093.
3. Revell PA. 2005. J. Immunol. 2005. 174:2124.
4. Kelso A. 2002. Int. Immunol. 6:605.
5. Getachew Y, et al. 2008. J. Immunol. 181:7810.
6. Cai Sheng, et al. 2009. J. Immunol. 182:6287.

Gene ID
14940 View all products for this Gene ID
UniProt
View information about Granzyme C on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 04/27/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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