Loading...

PerCP/Cyanine5.5 anti-mouse/human CD44 Antibody

Pricing & Availability
Clone
IM7 (See other available formats)
Regulatory Status
RUO
Other Names
Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
IM7_PerCPCyanine55_CD44_Antibody_010319
C57BL/6 mouse splenocytes were stained with CD44 (clone IM7) PerCP/Cyanine5.5 (filled histogram) or rat IgG2b, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • IM7_PerCPCyanine55_CD44_Antibody_010319
    C57BL/6 mouse splenocytes were stained with CD44 (clone IM7) PerCP/Cyanine5.5 (filled histogram) or rat IgG2b, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
103031 25 µg £77
Check Availability


Need larger quantities of this item?
Request Bulk Quote
103032 100 µg £182
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus, Squirrel Monkey, Horse, Cow, Pig, Dog, Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Dexamethasone-induced myeloid leukemia M1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18 that is located between amino acids 145 and 18620. This clone has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, in vivo inhibition of DTH4,5, and spatial biology (IBEX)23,24. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Application References

(PubMed link indicates BioLegend citation)
  1. Trowbridge IS, et al. 1982. Immunogenetics 15:299. (ICFC, IP, CMCD)
  2. Katoh S, et al. 1994. J. Immunol. 153:3440. (ELISA)
  3. Budd RC, et al. 1987. J. Immunol. 138:3120. (IP)
  4. Camp RL, et al. 1993. J. Exp. Med. 178:497. (Block)
  5. Weiss JM, et al. 1997. J. Cell Biol. 137:1137. (Block)
  6. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) PubMed
  7. Cuff CA, et al. 2001. J. Clin. Invest. 108:1031. (IHC)
  8. Lee JW, et al. 2006. Nature Immunol. 8:181.
  9. Zhang N, et al. 2005. J. Immunol. 174:6967. PubMed
  10. Huabiao C, et al. 2005. J. Immunol. 175:591. PubMed
  11. Gui J, et al. 2007. Int. Immunol. 19:1201. PubMed
  12. Wang XY, et al. 2008. Blood 111:2436. PubMed
  13. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  14. Yamazaki J, et al. 2009. Blood PubMed
  15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  16. Chen YW, et al. 2010. Mol. Cancer Ther. 9:2879. PubMed
  17. Zheng Z, et al. 1995. J. Cell. Biol. 130:485.
  18. Wiranowska M, et al. 2010. Int. J. Cancer 127:532.
  19. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  20. Sandmaier BM, et al. 1998. Blood 91:3494.
  21. Yang Y, et al. 2015. Hypertension. 65:1047. PubMed
  22. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  23. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  24. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  2. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
  3. Schnell A, et al. 2021. Cell. 184:6281. PubMed
  4. Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed
  5. Paik D, et al. 2022. Nature. 603:907. PubMed
  6. Chakraborty P, et al. 2022. Cancer Res. 82:1969. PubMed
  7. Chae CS, et al. 2022. Cancer Discov. 12:1904. PubMed
  8. Dölz M, et al. 2022. iScience. 25:105372. PubMed
  9. Edwards SC, et al. 2023. J Exp Med. 220: . PubMed
  10. Sieow JL, et al. 2023. Int J Mol Sci. 24: . PubMed
  11. Kasuya T, et al. 2023. Sci Rep. 13:1653. PubMed
  12. Sun Y, et al. 2023. Cell Mol Life Sci. 80:63. PubMed
  13. Nanishi E, et al. 2023. NPJ Vaccines. 8:18. PubMed
  14. Du J, et al. 2022. Sci Immunol. 7:eabo5407. PubMed
  15. Zhu C, et al. 2022. Chemotherapy. 67:211. PubMed
  16. Fan NW, et al. 2023. FASEB J. 37:e22855. PubMed
  17. Battistello E, et al. 2023. Mol Cell. 83:1216. PubMed
  18. Fu S, et al. 2023. Nat Commun. 14:2248. PubMed
  19. Corria-Osorio J, et al. 2023. Nat Immunol. 24:869. PubMed
  20. Schmitt E, et al. 2013. J Immunol. 191:5640. PubMed
  21. Chen H, et al. 2005. J Immunol. 175:591. PubMed
  22. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  23. Gerber AN, et al. 2021. Cell Rep. 37:109816. PubMed
  24. Moudra A, et al. 2021. J Immunol. 206:2109. PubMed
  25. Webb LMC, et al. 2021. Aging Cell. 20:e13295. PubMed
  26. Bromley SK, et al. 2020. Cell Reports. 32(9):108085. PubMed
  27. Fernandes RA, et al. 2020. eLife. 9:e58463.. PubMed
  28. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 5:64. PubMed
  29. Park JH, et al. 2022. STAR Protoc. 3:101607. PubMed
  30. Amir M, et al. 2018. Cell Rep. 25:3733. PubMed
  31. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  32. Papafragkos I, et al. 2022. Front Immunol. 13:889075. PubMed
  33. Fumagalli V, et al. 2020. J Exp Med. :217. PubMed
  34. Zhang H, et al. 2020. Cancer Cell. 37(1):37-54.e9.. PubMed
  35. Silva M, et al. 2021. Sci Immunol. 6:eabf1152. PubMed
  36. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  37. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  38. Cong M, et al. 2021. Methods Mol Biol. 2388:59. PubMed
  39. Zhang P, et al. 2022. BMC Med. 20:435. PubMed
  40. Smith AS, et al. 2022. J Immunother Cancer. 10:. PubMed
  41. Cassidy BR, et al. 2020. J Neuroinflammation. 17:259. PubMed
  42. Jungwirth N et al. 2018. Journal of molecular neuroscience : MN. 65(1):60-73 . PubMed
  43. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  44. Wang J, et al. 2021. Nat Commun. 12:6198. PubMed
  45. Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed
  46. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  47. Jing Y, et al. 2020. Sci Adv. 6:eaax9455. PubMed
  48. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  49. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  50. Nakajima-Adachi H, et al. 2017. PLoS One. 12(2):e0172795. PubMed
  51. Vaena S, et al. 2021. Cell Reports. 35(5):109076. PubMed
  52. Horkova V, et al. 2020. Cell Reports. 30(5):1504-1514.e7.. PubMed
  53. Chauveau L, et al. 2021. EMBO Rep. 22:e52447. PubMed
  54. Webb ER, et al. 2022. iScience. 25:104995. PubMed
  55. Walsh SM, et al. 2021. eLife. 10:00. PubMed
  56. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  57. Brown SM et al. 2017. Endocrinology. 158(10):3592-3604 . PubMed
  58. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  59. Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed
  60. Watson MJ, et al. 2021. Nature. 591:645. PubMed
  61. Dunn JLM, et al. 2021. Mucosal Immunol. 14:1133. PubMed
  62. Chen RJ, et al. 2022. iScience. 25:105595. PubMed
  63. Nabet BY et al. 2017. Cell. 170(2):352-366 . PubMed
  64. Benci JL et al. 2019. Cell. 178(4):933-948 . PubMed
  65. Lee-Chang C, et al. 2021. J Exp Med. 218:. PubMed
  66. Sun Z, et al. 2013. PLoS One. 8:74672. PubMed
  67. Lu YJ, et al. 2021. Cell Rep. 36:109696. PubMed
  68. Benci JL et al. 2016. Cell. 167(6):1540-1554 . PubMed
  69. Nanishi E, et al. 2022. Commun Biol. 5:790. PubMed
  70. Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed
  71. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 0.252777778. PubMed
  72. Dhayade S, et al. 2020. Nutrients. 12:. PubMed
  73. Delmas A, et al. 2016. PLoS One. 11: 0163305. PubMed
  74. Charlton J, et al. 2015. PLoS One. 10:119200. PubMed
  75. Chakraborty P, et al. 2020. Cell Reports. 28(7):1879-1893.e7.. PubMed
  76. Fleskens V, et al. 2019. Cell Rep. 26:3600. PubMed
  77. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  78. Parigger T, et al. 2021. Int J Mol Sci. 22:. PubMed
  79. Goubet AG, et al. 2021. Cell Death Differ. 28:2276. PubMed
  80. Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed
  81. Qi S, et al. 2020. Theranostics. 10:1814. PubMed
RRID
AB_2076204 (BioLegend Cat. No. 103031)
AB_2076204 (BioLegend Cat. No. 103032)

Antigen Details

Structure
Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution

All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells

Function
Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor
Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type
B cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.
5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
6. Liu T, et al. 2009. Biol. Direct 4:40.

Gene ID
12505 View all products for this Gene ID 960 View all products for this Gene ID
UniProt
View information about CD44 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All CD44 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse/human CD44 IM7 FC
Biotin anti-mouse/human CD44 IM7 FC,ICC,IHC-P
FITC anti-mouse/human CD44 IM7 FC,ICC,IHC-P
PE/Cyanine5 anti-mouse/human CD44 IM7 FC
Purified anti-mouse/human CD44 IM7 FC,IHC-F,CyTOF®,ELISA,ICC,IHC-P,IP,CMCD,Stim
Brilliant Violet 605™ anti-mouse/human CD44 IM7 FC
PE anti-mouse/human CD44 IM7 FC
Alexa Fluor® 488 anti-mouse/human CD44 IM7 FC,ICC,IHC-P,SB
Alexa Fluor® 647 anti-mouse/human CD44 IM7 FC,ICC,SB
Pacific Blue™ anti-mouse/human CD44 IM7 FC
Alexa Fluor® 700 anti-mouse/human CD44 IM7 FC,SB
PE/Cyanine7 anti-mouse/human CD44 IM7 FC
APC/Cyanine7 anti-mouse/human CD44 IM7 FC
PerCP/Cyanine5.5 anti-mouse/human CD44 IM7 FC
PerCP anti-mouse/human CD44 IM7 FC
Brilliant Violet 421™ anti-mouse/human CD44 IM7 FC,ICC,IHC-P
Brilliant Violet 570™ anti-mouse/human CD44 IM7 FC
Brilliant Violet 785™ anti-mouse/human CD44 IM7 FC
Brilliant Violet 510™ anti-mouse/human CD44 IM7 FC
Ultra-LEAF™ Purified anti-mouse/human CD44 IM7 FC,CyTOF®,ELISA,IHC,IP,CMCD,Stim
Brilliant Violet 650™ anti-mouse/human CD44 IM7 FC
Purified anti-mouse/human CD44 (Maxpar® Ready) IM7 FC,CyTOF®
Alexa Fluor® 594 anti-mouse/human CD44 IM7 ICC,FC,IHC-P,SB
PE/Dazzle™ 594 anti-mouse/human CD44 IM7 FC
Brilliant Violet 711™ anti-mouse/human CD44 IM7 FC
APC/Fire™ 750 anti-mouse/human CD44 IM7 FC
TotalSeq™-A0073 anti-mouse/human CD44 IM7 PG
TotalSeq™-C0073 anti-mouse/human CD44 IM7 PG
TotalSeq™-B0073 anti-mouse/human CD44 IM7 PG
Spark YG™ 570 anti-mouse/human CD44 IM7 FC,IHC-P
Spark YG™ 593 anti-mouse/human CD44 IM7 FC
TotalSeq™-D0073 anti-mouse/human CD44 IM7 PG
Brilliant Violet 750™ anti-mouse/human CD44 IM7 FC
PerCP/Fire™ 806 anti-mouse/human CD44 IM7 FC
Spark Red™ 718 anti-mouse/human CD44 IM7 FC
PE/Fire™ 810 anti-mouse/human CD44 IM7 FC
Spark Blue™ 550 anti-mouse/human CD44 (Flexi-Fluor™) IM7 FC
Spark Red™ 718 anti-mouse/human CD44 (Flexi-Fluor™) IM7 FC
Go To Top Version: 4    Revision Date: 01/03/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account