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PerCP/Cyanine5.5 anti-mouse IgD Antibody

Pricing & Availability
Clone
11-26c.2a (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin D
Isotype
Rat IgG2a, κ
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Product Citations
publications
11-26Cdot2a_PCPCy55_030209.jpg
C57BL/6 splenocytes stained with 11-26c.2a PerCP/Cyanine5.5
  • 11-26Cdot2a_PCPCy55_030209.jpg
    C57BL/6 splenocytes stained with 11-26c.2a PerCP/Cyanine5.5
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
405709 25 µg £97
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405710 100 µg £238
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Description

Surface IgD is an important B cell differentiation marker.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3, and spatial biology (IBEX)10,11.

Application References

(PubMed link indicates BioLegend citation)
  1. Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
  2. Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
  3. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
  4. Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
  5. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  6. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
  7. Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
  8. Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
  9. Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed
  2. Guilbert C, et al. 2020. Curr Protoc Toxicol. 83:e91. PubMed
  3. Knudsen ML, et al. 2022. iScience. 25:105473. PubMed
  4. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  5. Kelly A, et al. 2013. Toxicol Sci. 131:434. PubMed
  6. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  7. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  8. Komban RJ, et al. 2019. Nat Commun. 10:2423. PubMed
  9. Lin YR, et al. 2020. Immunity. 53(4):840-851.e6. PubMed
  10. Parks KR, et al. 2019. Cell Rep. 29:3060. PubMed
  11. Na B, et al. 2016. PLoS One. 11: 0156429. PubMed
  12. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  13. Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed
  14. Torres-Ruiz J, et al. 2021. Front Immunol. 12:689966. PubMed
  15. Duan L, et al. 2021. Immunity. 54:2256. PubMed
  16. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  17. Hutter K, et al. 2020. FEBS J. . PubMed
  18. Zhu Y, et al. 2022. Clin Transl Med. 12:e887. PubMed
  19. Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed
  20. Bemark M, et al. 2016. Nat Commun. 7: 12698. PubMed
  21. Chauveau L, et al. 2021. EMBO Rep. 22:e52447. PubMed
  22. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  23. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  24. He Z, et al. 2016. Exp Hematol. 44:161-165. PubMed
  25. Yin S et al. 2019. Cancer cell. 35(2):283-296 . PubMed
  26. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  27. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  28. Cantor DJ et al. 2019. Cell reports. 26(1):108-118 . PubMed
  29. Prüss H, et al. 2017. Nat Neurosci. 10.1038/nn.4643. PubMed
  30. Sabouri Z, et al. 2016. Nat Commun. 7:13381. PubMed
  31. Monzon-Casanova E, et al. 2021. Eur J Immunol. 51:2266. PubMed
  32. Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed
  33. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
RRID
AB_1575115 (BioLegend Cat. No. 405709)
AB_1575115 (BioLegend Cat. No. 405710)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation
Cell Type
B cells
Biology Area
Immunology
Gene ID
380797 View all products for this Gene ID
UniProt
View information about IgD on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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