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PerCP/Cyanine5.5 anti-mouse IgM Antibody

Pricing & Availability
Clone
RMM-1 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin M
Isotype
Rat IgG2a, κ
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Product Citations
publications
RMM-1_PerCPCyanine55_IgM_Antibody_010319
C57BL/6 splenocytes were stained with anti-mouse IgM (clone RMM-1) PerCP/Cyanine5.5 (filled histogram) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • RMM-1_PerCPCyanine55_IgM_Antibody_010319
    C57BL/6 splenocytes were stained with anti-mouse IgM (clone RMM-1) PerCP/Cyanine5.5 (filled histogram) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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406511 25 µg £101
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406512 100 µg £185
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Description

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.

Application References

(PubMed link indicates BioLegend citation)
  1. Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed
Product Citations
  1. Xu Y, et al. 2022. Nat Commun. 13:980. PubMed
  2. Grégoire C, et al. 2022. Immunity. 55:1216. PubMed
  3. Minott JA, et al. 2022. Front Immunol. 13:1038340. PubMed
  4. Fontana MF, et al. 2023. Elife. 12: . PubMed
  5. Kim CC et al. 2019. Cell Rep. 27(5):1446-1460 . PubMed
  6. Lee JH, et al. 2018. Transgenic Res. 27:241. PubMed
  7. Zhang Z, et al. 2021. Oncoimmunology. 10:1912472. PubMed
  8. Gibb D, et al. 2011. J Immunol. 186:4244. PubMed
  9. Nelson SA, et al. 2022. NPJ Vaccines. 7:124. PubMed
  10. Patrussi L, et al. 2019. Haematologica. 104:2040. PubMed
  11. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  12. Li D, et al. 2022. Emerg Microbes Infect. 11:2248. PubMed
  13. Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed
  14. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  15. Werner A, et al. 2021. iScience. 24:103076. PubMed
  16. Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed
  17. Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
  18. Ang QY, et al. 2020. Cell. 181(6):1263-1275.e16. PubMed
  19. Whiteley AM et al. 2017. eLife. 6 pii: e26435. PubMed
  20. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
RRID
AB_2075943 (BioLegend Cat. No. 406511)
AB_2075943 (BioLegend Cat. No. 406512)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type
B cells
Biology Area
Immunology
Gene ID
16019 View all products for this Gene ID
UniProt
View information about IgM on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 01/03/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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