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PerCP/Cyanine5.5 anti-mouse Siglec H Antibody

Pricing & Availability
Clone
551 (See other available formats)
Regulatory Status
RUO
Other Names
Sialic acid-binding immunoglobulin-like lectin, SiglecH
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
551_PerCPCyanine55_Siglec-H_Antibody_1_110624
C57BL/6 splenocytes stained with CD45R/B220 (clone RA3-6B2) Alexa Fluor® 647 and Siglec H (clone 551) PerCP/Cyanine5.5 (left) or Rat IgG1, κ isotype control (right).
  • 551_PerCPCyanine55_Siglec-H_Antibody_1_110624
    C57BL/6 splenocytes stained with CD45R/B220 (clone RA3-6B2) Alexa Fluor® 647 and Siglec H (clone 551) PerCP/Cyanine5.5 (left) or Rat IgG1, κ isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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129613 25 µg £85
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129614 100 µg £230
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Description

The Siglec-H is a member of CD33-related Siglec family which mostly expressed in the innate immune system. Siglec-H is reported to be specifically expressed on plasmacytoid dendritic cells. Siglec-H depends on adaptor protein DAP 12 (DNAX-activating protein of 12 kD) for surface expression and signal transduction. Cross-linking with this antibody can selectively inhibit interferon-alpha production by IPCs following TLR9 ligation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Siglec-H-Fc recombinant fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated withPerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Blasius AL, et al. 2006. J. Immunol. 177:3260.
Product Citations
  1. Du Y, et al. 2022. J Exp Med. 219: . PubMed
  2. Feng J, et al. 2022. Immunity. 55:405. PubMed
  3. Peng J, et al. 2023. Am J Reprod Immunol. 89:e13678. PubMed
  4. Liao Y, et al. 2017. Virol J. 10.1186/s12985-017-0801-x. PubMed
  5. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  6. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  7. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  8. Lee HY, et al. 2021. Curr Protoc. 1:e272. PubMed
RRID
AB_10639936 (BioLegend Cat. No. 129613)
AB_10639936 (BioLegend Cat. No. 129614)

Antigen Details

Structure
Sialic acid-binding immunoglobulin-like lectins
Distribution

Siglec-H is expressed exclusively on natural interferon-producing cells (IPCs), also called plasmacytoid dendritic cells in spleen, blood and lymph nodes. The IPCs are characterized by a complex phenotype (CD11c+, Ly-6C+, B220+, CD11b-, CD8+/-).

Function
Siglec-H associates with adaptor protein DAP12 for cell signaling, which reduces type 1 IFN response of IPC to CpG.
Ligand/Receptor
Sialic acid
Cell Type
Dendritic cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Siglec Molecules
Antigen References

1. Blasius AL, et al. 2006. Blood 107:2474.
2. Zhang J, et al. 2006. Blood 107:3600.
3. Blasius AL, et al. 2007. J. Exp. Med. 204:2561.

Gene ID
233274 View all products for this Gene ID
UniProt
View information about Siglec H on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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