PerCP/Cyanine5.5 anti-rat CD45 Antibody anti-CD45

Pricing & Availability

Clone: OX-1 (See other available formats)
Regulatory Status: RUO
Other Names: Leukocyte common antigen, LCA, T200, Ly-5
Isotype: Mouse IgG1, κ
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Product Citations: publications

Ox-1_PerCPCy5.5_102110 — LOU rat splenocytes were stained with rat CD45 (clone OX-1) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).

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202220

100 µg

£238

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Description

CD45 is a 180-220 kD protein also known as leukocyte common antigen (LCA). It is a protein tyrosine phosphatase with multiple isoforms differing as a result of alternative splicing of the extracellular domain and glycosylation. CD45 is expressed on all hematopoietic cells except erythrocytes and platelets; isoform expression depends on cell type, activation state, and cell maturation. CD45 functions in signal transduction through T and B cell antigen receptors. CD45 has been shown to interact with various proteins including galectin-1, CD2, CD3, and CD4. The OX-1 antibody has been shown to partially inhibit NK cell-mediated lysis of syngeneic tumor cells in vitro.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Enriched glycoprotein fraction from Wistar rat thymocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunofluorescence microscopy (acetone fixed, ammonium-thiocyanate separated epidermal sheets)², immunohistochemistry of acetone³-, isopentane-⁴, PLP⁵-, and n-hexane⁶ fixed frozen sections and paraffin-embedded sections^7,8, and partial inhibition of NK cell lysis of syngeneic tumor cell lines¹.
RRID: AB_10683172 (BioLegend Cat. No. 202220)

Antigen Details

Structure: Protein tyrosine phosphatase, type I transmembrane protein, 180-220 kD (multiple isoforms and differential glycosylation)
Distribution: All hematopoietic cells except erythrocytes and platelets
Function: T cell receptor and B cell receptor signal transduction
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
Molecular Family: CD Molecules
Antigen References: 1. Sunderland CA, et al. 1979. Eur. J. Immunol. 9:155.
2. Woolett GR, et al. 1985. Eur. J. Immunol.. 15:168.
Gene ID: 24699 View all products for this Gene ID
UniProt: View information about CD45 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All CD45 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-rat CD45	OX-1	FC,IHC-P,IHC-F,IP
Biotin anti-rat CD45	OX-1	FC
FITC anti-rat CD45	OX-1	FC
PE anti-rat CD45	OX-1	FC
APC/Cyanine7 anti-rat CD45	OX-1	FC
PE/Cyanine7 anti-rat CD45	OX-1	FC
PerCP/Cyanine5.5 anti-rat CD45	OX-1	FC
Alexa Fluor® 488 anti-rat CD45	OX-1	FC
Alexa Fluor® 647 anti-rat CD45	OX-1	FC
Alexa Fluor® 700 anti-rat CD45	OX-1	FC
APC/Fire™ 750 anti-rat CD45	OX-1	FC
Pacific Blue™ anti-rat CD45	OX-1	FC
PE/Dazzle™ 594 anti-rat CD45	OX-1	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

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