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PerCP/Cyanine5.5 anti-mouse CD90.2 (Thy1.2) Recombinant Antibody

Pricing & Availability
Clone
QA20B09 (See other available formats)
Regulatory Status
RUO
Other Names
THY-1.2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA20B09_PerCPCyanine55_CD90-2_Antibody_1_101123
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε FITC and anti-mouse CD90.2 (Thy1.2) recombinant (clone QA20B09) PerCP/Cyanine5.5 (left) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
  • QA20B09_PerCPCyanine55_CD90-2_Antibody_1_101123
    C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε FITC and anti-mouse CD90.2 (Thy1.2) recombinant (clone QA20B09) PerCP/Cyanine5.5 (left) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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117109 25 µg £72
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117110 100 µg £137
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Description

CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. 

Excitation Laser
Blue Laser (488 nm)
RRID
AB_3097242 (BioLegend Cat. No. 117109)
AB_3097242 (BioLegend Cat. No. 117110)

Antigen Details

Structure
IgG superfamily, 25-35 kD
Distribution

Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice

Function
Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells
Cell Type
Neurons, T cells, Thymocytes
Biology Area
Adaptive Immunity, Cell Biology, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
  2. Craig W, et al. 1993. J Exp Med. 177:1331-42.
  3. Reif AE and Schlesinger M. 1989. Cell Surface Antigen Thy-1.
  4. Mayani H, et al. 1994. Blood. 83:2410-7.
Gene ID
21838 View all products for this Gene ID
UniProt
View information about CD90.2 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 10/11/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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